M-001 Dual Channel Serial Injection - Analyst with ICC-CE
From LEAP
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Current revision (20:19, 28 January 2010) (view source) (→For other cost/time saving measures consider:) |
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=== Overview === | === Overview === | ||
| - | This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within | + | This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within [https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo '''Analyst® Software'''] from ''Applied Biosystems Inc® '' using a LEAP Cycle [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php (requires ICC-CE software)]. |
| - | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for | + | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for [http://leapwiki.com/mediawiki/index.php?title=Category:Regeneration regeneration]. This configuration requires a 6 port [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/6-port-2-position-valve-155.php valve] for sample loading and a 10 port [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/10-port-2-position-valve-157.php valve]for column switching. Both valves must be controlled by the LEAP cycle. |
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| - | | Saving of Reagent time | + | | Saving of Reagent time [http://www.leapwiki.com/mediawiki/index.php?title=Category:Backcut (Back cut)] |
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| Increase throughput at little cost | | Increase throughput at little cost | ||
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| - | | Cannot do | + | | Cannot do [http://www.leapwiki.com/mediawiki/index.php?title=Category:Look_Ahead "Look Ahead"] |
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| - | | Injections are not | + | | Injections are not [http://www.leapwiki.com/mediawiki/index.php?title=Category:Staggered_Injections staggered] |
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| Peaks of interest must come off early | | Peaks of interest must come off early | ||
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=== For other cost/time saving measures consider:=== | === For other cost/time saving measures consider:=== | ||
| - | * LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title= | + | * [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve] |
| - | + | * [http://www.leapwiki.com/mediawiki/index.php?title=M-002_Dual_Channel_Serial_Injection_with_front_cut_-_Analyst_with_ICC-CE Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE ] | |
| - | + | * [http://www.leapwiki.com/mediawiki/index.php?title=M-003_Dual_Channel_Parallel_Injection_-_Cycle_Composer Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst] | |
| - | + | * [http://www.leaptec.com/software/leap-shell-3.php “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software] | |
| - | + | * [http://www.leapwiki.com/mediawiki/index.php?title=M-005_Dual_Channel_Parallel_Injection_-_LEAP_Shell_%22(M-Fab)%22 Dual Channel Parallel Injection (M-005) using LEAP Shell software] | |
| - | + | * [http://www.leapwiki.com/mediawiki/index.php?title=Summary_of_LC_Multi_Valve_Applications Summary of LC Multi Valve Applications ] | |
| - | + | * [http://www.leaptec.com/software/leap-shell-3.php LEAP Shell Software and application for custom injections and scheduling ] | |
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| + | === Accessories for the PAL === | ||
| + | [[image:Accessories_Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Category:Accessories Other Accessories for PAL Robots] | ||
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]] | [[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]] | ||
Current revision
| LC Column Switching & Regeneration |
| Application Type | |
| SPECIAL | |
| Application ID | |
| M-001 | |
| Description | |
| LC Column Switching & Regeneration. |
Contents |
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst® Software from Applied Biosystems Inc® using a LEAP Cycle (requires ICC-CE software).
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve for sample loading and a 10 port valvefor column switching. Both valves must be controlled by the LEAP cycle.
Please note: LEAP can not control the MS Selector valve as part of the cycle.
Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system
| Key Benefits |
| Saving of Reagent time (Back cut) |
| Increase throughput at little cost |
| Control is all within Analyst Software |
| Compliance not affected |
| GLP Compliant |
| Restrictions |
| Cannot do "Look Ahead" |
| Injections are not staggered |
| Peaks of interest must come off early |
| Columns may perform slightly different on alternating samples |
| Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
|---|---|---|---|---|---|---|
| M-001 | Dual Channel Serial Injection Provides shorter cycle times by regenerating columns during next analytical run | Analyst with ICC-CE | * 6 port injection valve * 10 port Valve * Gradient Pump * Regeneration pump | * Saving of regeneration time(Back Cut) * Increase throughput at little cost * Control is all within Analyst * Compliance is not affected | * Can not do "Look Ahead" * Injections are not staggered * Peaks of interest must come of early * Columns may perform slightly differently on alternating samples | Yes |
Typical System of PAL with CoolStack Fast Wash and Valves
When columns must be regenerated following each analysis, this technique permits automation of the process. While one column performs the analysis, the second column undergoes regeneration by use of an auxiliary pump. Once the first analysis is complete, the valve is switched and the regenerated column is now ready for analytical use.
PRINT Function for Page
PDF Summary of Upgrade to Existing System
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software
- Dual Channel Parallel Injection (M-005) using LEAP Shell software
- Summary of LC Multi Valve Applications
- LEAP Shell Software and application for custom injections and scheduling
Accessories for the PAL
Other Accessories for PAL Robots
This workstation application is based on the CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your LC or GC requirements. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible automation.
Contact LEAP
Contact the LEAP office
Keywords: Dual Channel Serial Injection, Provides shorter cycle times by regenerating columns during next analytical run, Analyst with ICC-CE, 6 port injection valve, 10 port Valve, Gradient Pump ,Regeneration pump, Saving of regeneration time, Back Cut, Increase throughput at little cost, Control is all within Analyst, Compliance is not affected, Can not do Look Ahead, Injections are not staggered, Peaks of interest must come of early, Columns may perform slightly differently on alternating samples, ICC-CE software, cycles
