MS Tuning

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LC Column Switching & Regeneration
Application Type
  SPECIAL
Application ID
  M-001
Description
  LC Column Switching & Regeneration.
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Overview

This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst® Software[1] from Applied Biosystems Inc® using a LEAP Cycle (requires ICC-CE software) [2].

There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[3] for sample loading and a 10 port valve [4]for column switching. Both valves must be controlled by the LEAP cycle.

Please note: LEAP can not control the MS Selector valve as part of the cycle.

Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system
Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system





Key Benefits
Saving of Reagent time (Back cut)
Increase throughput at little cost
Control is all within Analyst Software
Compliance not affected
GLP Compliant



Restrictions
Cannot do "Look Ahead"
Injections are not staggered
Peaks of interest must come off early
Columns may perform slightly different on alternating samples








Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-001 Dual Channel Serial Injection

Provides shorter cycle times by regenerating columns during next analytical run
Analyst with ICC-CE * 6 port injection valve
* 10 port Valve
* Gradient Pump
* Regeneration pump
* Saving of regeneration time(Back Cut)
* Increase throughput at little cost
* Control is all within Analyst
* Compliance is not affected
* Can not do "Look Ahead"
* Injections are not staggered
* Peaks of interest must come of early
* Columns may perform slightly differently on alternating samples
Yes

Typical system for Dual Column with Regeneration


For other cost/time saving measures consider:

  • LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [5]
  • Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [6]
  • Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [7]
  • “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [8]
  • Dual Channel Parallel Injection (M-005) using LEAP Shell software [9]
  • LEAP Shell Software and application for custom injections and scheduling [10]

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www.leaptec.com
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Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information



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