MS Tuning

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(New page: {{LSApp |name = LC Column Switching & Regeneration |image = M-001.png |type = '''SPECIAL''' |id = M-001 |description = LC Column Switching & Regene...)
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{{LSApp
{{LSApp
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   |name        = LC Column Switching & Regeneration
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   |name        = Mass Spec Tuning and column selection
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   |image      = M-001.png  
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   |image      = Tuning-PlumbDiag.png
   |type        = '''SPECIAL'''
   |type        = '''SPECIAL'''
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   |id          = M-001
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   |id          = Project Ref: H1108
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   |description = LC Column Switching & Regeneration.
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   |description = MS tuning
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[[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]]
[[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]]
=== Overview ===
=== Overview ===
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This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within '''Analyst®  Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc® '' using a LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php].
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This configuration allows MS tuning and column selection. This is run directly from within '''Analyst®  Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc® '' using a LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php].
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There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/6-port-2-position-valve-155.php] for sample loading and a 10 port valve [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/10-port-2-position-valve-157.php]for column switching. Both valves must be controlled by the LEAP cycle.  
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This configuration requires a 6 port valve[http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/6-port-2-position-valve-155.php] for sample loading of the MS tuning and a TrioVS valve for LEAP created analytical cycles. All valves must be controlled by the LEAP cycle.  
''Please note: LEAP can not control the MS Selector valve as part of the cycle.''
''Please note: LEAP can not control the MS Selector valve as part of the cycle.''
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[[Image:M-001.png|frame|Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system]]
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There are four LEAP created Analyst cycles.  
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<br><br><br><br>
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The first LEAP created cycle is known as a Tuning cycle. It takes samples and passes them directly to the Mass Spec through the stand-alone 6-port LC Valve. By directly, it is meant to emphasize that the sample does not pass through any column whatsoever during its passage to the Mass Spec.
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{| border="1"
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The second and third LEAP created cycle Analyst cycles inject the sample to the top valve of the TrioVS. The difference between the two cycles is that one passes the sample through column A (connected to ports 10 and 7 of the 2nd Valve of the TrioVS) on its way to the Mass Spec, while the other cycle passes the sample through column B (connected to ports 2 and 5 of the 2nd Valve of the TrioVS). 
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| ''' Key Benefits '''
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| Saving of Reagent time (Back cut)
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| Increase throughput at little cost
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| Control is all within Analyst Software
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| Compliance not affected
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| GLP Compliant
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<br><br>
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{| border="1"
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| '''Restrictions'''
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| Cannot do "Look Ahead"
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| Injections are not staggered
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| Peaks of interest must come off early
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| Columns may perform slightly different on alternating samples
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<br><br><br><br><br><br>
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The final LEAP created cycle Analyst cycle is used for a single-column application. In this scenario, the second valve of the TrioVS is bypassed altogether. The column will be included in the plumbing lines connecting the 6-port valve of the TrioVS to the selector valve of the TrioVS.
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{| cellpadding="6" style="border:1px solid darkgray;"
 
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|- align=left
 
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!width="20"|Code
 
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!width="195"|Application
 
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!width="85"|Control SW
 
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!width="140"|Key Hardware
 
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!width="205"|Key Benefits
 
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!width="235"|Restrictions
 
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!width="15"|GLP
 
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|- align=left
 
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| style="border:1px solid blue;"|'''M-001'''
 
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| style="border:1px solid blue;"|'''Dual Channel Serial Injection'''<br><br>Provides shorter cycle times by regenerating columns during next analytical run
 
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| style="border:1px solid blue;"|Analyst with ICC-CE
 
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| style="border:1px solid blue;"|* 6 port injection valve<BR>* 10 port Valve <br>* Gradient Pump<br>* Regeneration pump
 
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| style="border:1px solid blue;"|* Saving of regeneration time(Back Cut)<br>* Increase throughput at little cost<br> * Control is all within Analyst<br>* Compliance is not affected
 
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| style="border:1px solid blue;"|* Can not do "Look Ahead"<br>* Injections are not staggered<br>* Peaks of interest must come of early<br>* Columns may perform slightly differently on alternating samples
 
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| style="border:1px solid blue;"|Yes
 
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[[Image:M-001_HTC_CoolStack.png|Typical system for Dual Column with Regeneration]]
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[[Image:Tuning-PlumbDiag.png]]
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Revision as of 02:39, 29 April 2009

Mass Spec Tuning and column selection
Application Type
  SPECIAL
Application ID
  Project Ref: H1108
Description
  MS tuning
www.leaptec.comwww.leapwiki.com
www.leaptec.com
www.leapwiki.com

Overview

This configuration allows MS tuning and column selection. This is run directly from within Analyst® Software[1] from Applied Biosystems Inc® using a LEAP Cycle (requires ICC-CE software) [2].

This configuration requires a 6 port valve[3] for sample loading of the MS tuning and a TrioVS valve for LEAP created analytical cycles. All valves must be controlled by the LEAP cycle.

Please note: LEAP can not control the MS Selector valve as part of the cycle.

There are four LEAP created Analyst cycles.

The first LEAP created cycle is known as a Tuning cycle. It takes samples and passes them directly to the Mass Spec through the stand-alone 6-port LC Valve. By directly, it is meant to emphasize that the sample does not pass through any column whatsoever during its passage to the Mass Spec.

The second and third LEAP created cycle Analyst cycles inject the sample to the top valve of the TrioVS. The difference between the two cycles is that one passes the sample through column A (connected to ports 10 and 7 of the 2nd Valve of the TrioVS) on its way to the Mass Spec, while the other cycle passes the sample through column B (connected to ports 2 and 5 of the 2nd Valve of the TrioVS).

The final LEAP created cycle Analyst cycle is used for a single-column application. In this scenario, the second valve of the TrioVS is bypassed altogether. The column will be included in the plumbing lines connecting the 6-port valve of the TrioVS to the selector valve of the TrioVS.


Image:Tuning-PlumbDiag.png


For other cost/time saving measures consider:

  • LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [4]
  • Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [5]
  • Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [6]
  • “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [7]
  • Dual Channel Parallel Injection (M-005) using LEAP Shell software [8]
  • LEAP Shell Software and application for custom injections and scheduling [9]
www.leaptec.comwww.leapwiki.com
www.leaptec.com
www.leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information



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