M-003 Dual Channel Parallel Injection - Cycle Composer
From LEAP
(→Hardware Requirements) |
|||
| Line 25: | Line 25: | ||
=== Hardware Requirements === | === Hardware Requirements === | ||
| - | * TrioVS multiple valve system is required | + | * TrioVS multiple valve system is required |
[[Image:Valve_Drawing_Dual_Stream_1.png|frame|Plumbing of valve diagram showing flow for each selected column]] | [[Image:Valve_Drawing_Dual_Stream_1.png|frame|Plumbing of valve diagram showing flow for each selected column]] | ||
| Line 76: | Line 76: | ||
|} | |} | ||
<br> | <br> | ||
| + | |||
=== For other cost/time saving measures consider:=== | === For other cost/time saving measures consider:=== | ||
* LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing] | * LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing] | ||
Revision as of 19:25, 29 April 2009
| LC Dual Stream Staggered Injections |
| Application Type | |
| SPECIAL | |
| Application ID | |
| M-003 | |
| Description | |
| High Throughput LC injections alternating onto two analytical columns. |
www.leapwiki.com
Contents |
Overview
This configuration allows shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating the alternating column during the acquisition. It uses
Cycle Composer Software[1] - this application is run with Standard LEAP macros outside of Analyst® Software[2] from Applied Biosystems Inc®
If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.
This application is useful to increase sample throughput by taking advantage of front and/or back-cut time to load the next sample in the sequence early. It requires that the "period of interest" in the chromatography is significantly shorter than the overall chromatographic run-time. There are two columns that are running in "staggered" mode, where samples are pre-loaded onto the other channel while the previous channel is still in acquisition. There is no pre-column. The gradient programs regenerate the analytical columns in each stream.
Please note: LEAP can not control the MS Selector valve as part of the cycle.
If injections are staggered in the following way, there is a potential for up to 100% gain:
Hardware Requirements
- TrioVS multiple valve system is required
| Key Benefits |
| Saving of Reagent time (Front and Back cut) |
| Performs "Look Ahead" |
| Staggering of Injections |
| Restrictions |
| Requires matching sample lists in Cycle Composer and Analyst |
| Peaks of interest must come off early |
| Columns may perform slightly different on alternating samples |
| Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
|---|---|---|---|---|---|---|
| M-003 | Dual Channel Parallel Injection Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition | Cycle Composer outside of Analyst | * Trio VS *6 port injection valve *FWS in front of 6 port valve * Gradient Pump * Regeneration pump | * Saving of Front and Back Cut * Provides "Look Ahead" *Provides staggering of injections | * Requires matching sample lists in Cycle Composer and Analyst * Columns may perform slightly differently on alternating samples | No |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [3]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [4]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [5]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [6]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [7]
- LEAP Shell Software and application for custom injections and scheduling [8]
www.leapwiki.com
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
