M-003 Dual Channel Parallel Injection - Cycle Composer

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If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.
If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.
This application is useful to increase sample throughput by taking advantage of front and/or back-cut time to load the next sample in the sequence early. It requires that the "period of interest" in the chromatography is significantly shorter than the overall chromatographic run-time. There are two columns that are running in "staggered" mode, where samples are pre-loaded onto the other channel while the previous channel is still in acquisition. There is no pre-column. The gradient programs regenerate the analytical columns in each stream.
This application is useful to increase sample throughput by taking advantage of front and/or back-cut time to load the next sample in the sequence early. It requires that the "period of interest" in the chromatography is significantly shorter than the overall chromatographic run-time. There are two columns that are running in "staggered" mode, where samples are pre-loaded onto the other channel while the previous channel is still in acquisition. There is no pre-column. The gradient programs regenerate the analytical columns in each stream.
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''Please note: LEAP can not control the MS Selector valve as part of the cycle.''
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Samples are processed in pairs, the application does NOT integrate with data systems, but contact closures are used to maintain synchronization with the detector.
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'''Benefit:''' Sample injection occurs onto each column in a staggered fashion and is offset by the acquisition time. Optimum sample throughput is achieved by sample injection onto column 1 during the front cut and/or column equilibration of column 2. Increased throughput is the end result, with up to 100% increase in time saved
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'''Equipment Requirements:'''
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* HTC PAL.
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* TrioVS Module
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* TrioValve 1 - LCVlv1 = Injection valve for both columns.
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* TrioValve 2 - LCVlv2 = Column selection valve.
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* TrioValve 3 - LCVlv3 = Stream selection valve
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* Two HPLC Pump systems
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[[Image:Front-backcut.png]]
[[Image:Front-backcut.png]]
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=== Hardware Requirements ===
=== Hardware Requirements ===
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* TrioVS multiple valve system is required in addition to the standard injection valve.
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* TrioVS multiple valve system is required
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[[Image:Valve_Drawing_Dual_Stream_1.png|frame|Plumbing of valve diagram showing flow for each selected column]]
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<br><br><br><br>
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{| border="1"
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| ''' Key Benefits '''
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{|
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| Saving of Reagent time (Front and Back cut)
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| Increase throughput at little cost
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<br><br>
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{| border="1"
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| '''Restrictions'''
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{|
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| Requires matching sample lists in Cycle Composer and Analyst
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| Peaks of interest must come off early
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| Columns may perform slightly different on alternating samples
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|}
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<br><br><br><br><br><br>
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[[Image:Valve_Drawing_Dual_Stream_1.png]]
 
{| cellpadding="6" style="border:1px solid darkgray;"
{| cellpadding="6" style="border:1px solid darkgray;"
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Revision as of 18:52, 29 April 2009

LC Dual Stream Staggered Injections
Application Type
  SPECIAL
Application ID
  M-003
Description
  High Throughput LC injections alternating onto two analytical columns.
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Contents

Overview

This configuration allows shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating the alternating column during the acquisition. It uses Cycle Composer Software[1] - this application is run with Standard LEAP macros outside of Analyst® Software[2] from Applied Biosystems Inc®

If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput. This application is useful to increase sample throughput by taking advantage of front and/or back-cut time to load the next sample in the sequence early. It requires that the "period of interest" in the chromatography is significantly shorter than the overall chromatographic run-time. There are two columns that are running in "staggered" mode, where samples are pre-loaded onto the other channel while the previous channel is still in acquisition. There is no pre-column. The gradient programs regenerate the analytical columns in each stream. Please note: LEAP can not control the MS Selector valve as part of the cycle.

Image:Front-backcut.png

If injections are staggered in the following way, there is a potential for up to 100% gain:


Image:Timeline_Dual_Stream_1.png


Hardware Requirements

  • TrioVS multiple valve system is required
Plumbing of valve diagram showing flow for each selected column
Plumbing of valve diagram showing flow for each selected column





Key Benefits
Saving of Reagent time (Front and Back cut)
Increase throughput at little cost



Restrictions
Requires matching sample lists in Cycle Composer and Analyst
Peaks of interest must come off early
Columns may perform slightly different on alternating samples







Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-003 Dual Channel Parallel Injection

Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition 		Cycle Composer outside of Analyst * Trio VS
*6 port injection valve
*FWS in front of 6 port valve
* Gradient Pump
* Regeneration pump 		* Saving of Front and Back Cut
* Provides "Look Ahead"
*Provides staggering of injections 		* Requires matching sample lists in Cycle Composer and Analyst
* Columns may perform slightly differently on alternating samples 		No


For other cost/time saving measures consider:

  • LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [3]
  • Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [4]
  • Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [5]
  • “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [6]
  • Dual Channel Parallel Injection (M-005) using LEAP Shell software [7]
  • LEAP Shell Software and application for custom injections and scheduling [8]
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www.leaptec.com
www.leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information




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