M-003 Dual Channel Parallel Injection - Cycle Composer
From LEAP
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* LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing] | * LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing] | ||
* Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-001_Dual_Channel_Serial_Injection_-_Analyst_with_ICC-CE] | * Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-001_Dual_Channel_Serial_Injection_-_Analyst_with_ICC-CE] | ||
Revision as of 23:17, 28 April 2009
| LC Dual Stream Staggered Injections |
| Application Type | |
| SPECIAL | |
| Application ID | |
| M-003 | |
| Description | |
| High Throughput LC injections alternating onto two analytical columns. |
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Contents |
Overview
This configuration allows shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating the alternating column during the acquisition. It uses
Cycle Composer Software[1] - this application is run with Standard LEAP macros outside of Analyst® Software[2] from Applied Biosystems Inc®
If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.
If injections are staggered in the following way, there is a potential for up to 100% gain:
Hardware Requirements
- TrioVS multiple valve system is required in addition to the standard injection valve.
| Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
|---|---|---|---|---|---|---|
| M-003 | Dual Channel Parallel Injection Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition | Cycle Composer outside of Analyst | * Trio VS *6 port injection valve *FWS in front of 6 port valve * Gradient Pump * Regeneration pump | * Saving of Front and Back Cut * Provides "Look Ahead" *Provides staggering of injections | * Requires matching sample lists in Cycle Composer and Analyst * Columns may perform slightly differently on alternating samples | No |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [3]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [4]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [5]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [6]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [7]
- LEAP Shell Software and application for custom injections and scheduling [8]
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Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
