M-002 Dual Channel Serial Injection with front cut - Analyst with ICC-CE
From LEAP
Current revision (10:55, 9 June 2009) (view source) (→Contact LEAP) |
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{{LSApp | {{LSApp | ||
- | |name = Dual Channel Serial Injection with | + | |name = Dual Channel Serial Injection with Front Cut |
- | |image = | + | |image = M-Fab_Photo1.png |
|type = '''SPECIAL''' | |type = '''SPECIAL''' | ||
- | |id = M- | + | |id = M-002 |
|description = High throughput where one column is regenerating while the other is performing analytical separation. An additional valve allows solvent front to be cut to waste. | |description = High throughput where one column is regenerating while the other is performing analytical separation. An additional valve allows solvent front to be cut to waste. | ||
}} | }} | ||
- | + | [[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]] | |
=== Overview === | === Overview === | ||
- | This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This | + | This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within '''Analyst Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc'' using a Standard LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php]. |
- | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a | + | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration [http://leapwiki.com/mediawiki/index.php?title=Category:Regeneration]. This configuration requires a TrioVS Valve [http://www.leaptec.com/products/lc-related-instruments-and-accessories/rotors-and-inlet-sleeves/pal-trio-vs--valve-system--80.php]. All of the valves must be controlled by the Standard LEAP cycle. <br> ''LEAP can not control the MS Selector valve as part of the cycle.'' |
+ | [[Image:Empty TRIO.png|frame|TrioVS valve is used. Flow path intentionally left incomplete. Contact LEAP for more information]] | ||
- | + | <br><br><br><br> | |
+ | {| border="1" | ||
+ | | ''' Key Benefits ''' | ||
+ | |} | ||
+ | {| | ||
+ | |- | ||
+ | | Saving of Reagent time (Back cut) [http://www.leapwiki.com/mediawiki/index.php?title=Category:Backcut] | ||
+ | |- | ||
+ | | Increase throughput at little cost | ||
+ | |- | ||
+ | | Control is all within Analyst Software | ||
+ | |- | ||
+ | | Compliance not affected | ||
+ | |- | ||
+ | | Avoids contaminating MS inlet with early eluting compounds and solvent | ||
+ | |} | ||
+ | <br><br> | ||
+ | {| border="1" | ||
+ | | '''Restrictions''' | ||
+ | |} | ||
+ | {| | ||
+ | |- | ||
+ | | Cannot do "Look Ahead"[http://www.leapwiki.com/mediawiki/index.php?title=Category:Look_Ahead] | ||
+ | |- | ||
+ | | Injections are not staggered[http://www.leapwiki.com/mediawiki/index.php?title=Category:Staggered_Injections] | ||
+ | |- | ||
+ | | Peaks of interest must come off early | ||
+ | |- | ||
+ | | Columns may perform slightly different on alternating samples | ||
+ | |- | ||
+ | | Requires TrioVS valve | ||
+ | |- | ||
+ | |} | ||
+ | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
+ | {| cellpadding="6" style="border:1px solid darkgray;" | ||
+ | |- align=left | ||
+ | !width="20"|Code | ||
+ | !width="195"|Application | ||
+ | !width="85"|Control SW | ||
+ | !width="140"|Key Hardware | ||
+ | !width="205"|Key Benefits | ||
+ | !width="235"|Restrictions | ||
+ | !width="15"|GLP | ||
+ | |- align=left | ||
+ | | style="border:1px solid blue;"|'''M-002''' | ||
+ | | style="border:1px solid blue;"|'''Dual Channel Serial Injection with Front Cut'''<br><br>Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste | ||
+ | | style="border:1px solid blue;"|Analyst with ICC-CE | ||
+ | | style="border:1px solid blue;"|* Trio VS<br>* Gradient Pump<br>* Regeneration pump | ||
+ | | style="border:1px solid blue;"|* Saving of regeneration time(Back Cut)<br>* Increase throughput at little cost<br> * Control is all within Analyst<br>* Compliance is not affected <br>* Avoids contaminating MS inlet with early eluting compounds and Solvent | ||
+ | | style="border:1px solid blue;"|* Can not do "Look Ahead"<br>* Injections are not staggered<br>* Peaks of interest must come of early<br>* Columns may perform slightly differently on alternating samples | ||
+ | | style="border:1px solid blue;"|Yes | ||
+ | |} | ||
- | [[Image: | + | === For other cost/time saving measures consider:=== |
+ | * LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing] | ||
+ | * Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-001_Dual_Channel_Serial_Injection_-_Analyst_with_ICC-CE] | ||
+ | * Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [http://www.leapwiki.com/mediawiki/index.php?title=M-003_Dual_Channel_Parallel_Injection_-_Cycle_Composer] | ||
+ | * “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [http://www.leaptec.com/software/leap-shell-3.php] | ||
+ | * Dual Channel Parallel Injection (M-005) using LEAP Shell software [http://www.leapwiki.com/mediawiki/index.php?title=M-005_Dual_Channel_Parallel_Injection_-_LEAP_Shell_%22(M-Fab)%22] | ||
+ | * Summary of LC Multi Valve Applications [http://www.leapwiki.com/mediawiki/index.php?title=Summary_of_LC_Multi_Valve_Applications] | ||
+ | * LEAP Shell Software and application for custom injections and scheduling [http://www.leaptec.com/software/leap-shell-3.php] | ||
+ | ---- | ||
+ | [[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]] | ||
+ | === Contact LEAP === | ||
+ | For additional information about this technique please contact LEAP Technologies for detailed information | ||
- | [[ | + | [[image:Contact_Info.png|40px]] [http://www.leaptec.com/contact-us.php Contact the LEAP office] |
+ | <br><br> | ||
+ | [[Category:Analyst with ICC-CE]] | ||
+ | [[Category:LC Multi Valve Applications]] |
Current revision
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration [3]. This configuration requires a TrioVS Valve [4]. All of the valves must be controlled by the Standard LEAP cycle.
LEAP can not control the MS Selector valve as part of the cycle.
Key Benefits |
Saving of Reagent time (Back cut) [5] |
Increase throughput at little cost |
Control is all within Analyst Software |
Compliance not affected |
Avoids contaminating MS inlet with early eluting compounds and solvent |
Restrictions |
Cannot do "Look Ahead"[6] |
Injections are not staggered[7] |
Peaks of interest must come off early |
Columns may perform slightly different on alternating samples |
Requires TrioVS valve |
Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
---|---|---|---|---|---|---|
M-002 | Dual Channel Serial Injection with Front Cut Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste | Analyst with ICC-CE | * Trio VS * Gradient Pump * Regeneration pump | * Saving of regeneration time(Back Cut) * Increase throughput at little cost * Control is all within Analyst * Compliance is not affected * Avoids contaminating MS inlet with early eluting compounds and Solvent | * Can not do "Look Ahead" * Injections are not staggered * Peaks of interest must come of early * Columns may perform slightly differently on alternating samples | Yes |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [8]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [9]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [10]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [11]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [12]
- Summary of LC Multi Valve Applications [13]
- LEAP Shell Software and application for custom injections and scheduling [14]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information