M-002 Dual Channel Serial Injection with front cut - Analyst with ICC-CE

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{{LSApp
{{LSApp
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   |name        = Dual Channel Serial Injection with front cut
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   |name        = Dual Channel Serial Injection with Front Cut
   |image      = M-Fab_Photo1.png
   |image      = M-Fab_Photo1.png
   |type        = '''SPECIAL'''
   |type        = '''SPECIAL'''
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   |id          = M-001
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   |id          = M-002
   |description = High throughput where one column is regenerating while the other is performing analytical separation. An additional valve allows solvent front to be cut to waste.
   |description = High throughput where one column is regenerating while the other is performing analytical separation. An additional valve allows solvent front to be cut to waste.
}}
}}
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
=== Overview ===
=== Overview ===
-
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This can be run directly from within '''Analyst''' using a custom Cycle (requires ICC-CE). With the addition of a selector valve, the solvent front can be cut to waste.
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This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within '''Analyst Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc'' using a Standard LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php].
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There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration [http://leapwiki.com/mediawiki/index.php?title=Category:Regeneration]. This configuration requires a TrioVS Valve [http://www.leaptec.com/products/lc-related-instruments-and-accessories/rotors-and-inlet-sleeves/pal-trio-vs--valve-system--80.php].  All of the valves must be controlled by the Standard LEAP cycle. <br> ''LEAP can not control the MS Selector valve as part of the cycle.''
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[[Image:Empty TRIO.png|frame|TrioVS valve is used. Flow path intentionally left incomplete. Contact LEAP for more information]]
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<br><br><br><br>
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{| border="1"
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| ''' Key Benefits '''
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|}
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{|
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|-
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| Saving of Reagent time (Back cut) [http://www.leapwiki.com/mediawiki/index.php?title=Category:Backcut]
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|-
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| Increase throughput at little cost
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|-
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| Control is all within Analyst Software
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|-
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| Compliance not affected
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|-
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| Avoids contaminating MS inlet with early eluting compounds and solvent
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|}
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<br><br>
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{| border="1"
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| '''Restrictions'''
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|}
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{|
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|-
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| Cannot do "Look Ahead"[http://www.leapwiki.com/mediawiki/index.php?title=Category:Look_Ahead]
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|-
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| Injections are not staggered[http://www.leapwiki.com/mediawiki/index.php?title=Category:Staggered_Injections]
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|-
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| Peaks of interest must come off early
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|-
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| Columns may perform slightly different on alternating samples
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|-
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| Requires TrioVS valve
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|-
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|}
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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{| cellpadding="6" style="border:1px solid darkgray;"
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|- align=left
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!width="20"|Code
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!width="195"|Application
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!width="85"|Control SW
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!width="140"|Key Hardware
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!width="205"|Key Benefits
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!width="235"|Restrictions
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!width="15"|GLP
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|- align=left
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| style="border:1px solid blue;"|'''M-002'''
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| style="border:1px solid blue;"|'''Dual Channel Serial Injection with Front Cut'''<br><br>Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste
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| style="border:1px solid blue;"|Analyst with ICC-CE
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| style="border:1px solid blue;"|* Trio VS<br>* Gradient Pump<br>* Regeneration pump
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| style="border:1px solid blue;"|* Saving of regeneration time(Back Cut)<br>* Increase throughput at little cost<br> * Control is all within Analyst<br>* Compliance is not affected <br>* Avoids contaminating MS inlet with early eluting compounds and Solvent
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| style="border:1px solid blue;"|* Can not do "Look Ahead"<br>* Injections are not staggered<br>* Peaks of interest must come of early<br>* Columns may perform slightly differently on alternating samples
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| style="border:1px solid blue;"|Yes
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|}
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=== For other cost/time saving measures consider:===
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* LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing]
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* Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-001_Dual_Channel_Serial_Injection_-_Analyst_with_ICC-CE]
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* Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [http://www.leapwiki.com/mediawiki/index.php?title=M-003_Dual_Channel_Parallel_Injection_-_Cycle_Composer]
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* “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [http://www.leaptec.com/software/leap-shell-3.php]
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* Dual Channel Parallel Injection (M-005) using LEAP Shell software [http://www.leapwiki.com/mediawiki/index.php?title=M-005_Dual_Channel_Parallel_Injection_-_LEAP_Shell_%22(M-Fab)%22]
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* Summary of LC Multi Valve Applications [http://www.leapwiki.com/mediawiki/index.php?title=Summary_of_LC_Multi_Valve_Applications]
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* LEAP Shell Software and application for custom injections and scheduling [http://www.leaptec.com/software/leap-shell-3.php]
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----
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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=== Contact LEAP ===
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 +
For additional information about this technique please contact LEAP Technologies for detailed information
-
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve for sample loading and a 10 port valve for column switching.<br> Both valves must be controlled by the custom cycle. LEAP can not control the MS Selector valve as part of the cycle.
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[[image:Contact_Info.png‎|40px]] [http://www.leaptec.com/contact-us.php Contact the LEAP office]
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<br><br>
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[[Category:Analyst with ICC-CE]]
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[[Category:LC Multi Valve Applications]]

Current revision

Dual Channel Serial Injection with Front Cut
Application Type
  SPECIAL
Application ID
  M-002
Description
  High throughput where one column is regenerating while the other is performing analytical separation. An additional valve allows solvent front to be cut to waste.
leaptec.comleapwiki.com
leaptec.com
leapwiki.com

Overview

This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput and allows solvent front to be cut to waste with a selector valve. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].

There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration [3]. This configuration requires a TrioVS Valve [4]. All of the valves must be controlled by the Standard LEAP cycle.
LEAP can not control the MS Selector valve as part of the cycle.

TrioVS valve is used. Flow path intentionally left incomplete. Contact LEAP for more information
TrioVS valve is used. Flow path intentionally left incomplete. Contact LEAP for more information





Key Benefits
Saving of Reagent time (Back cut) [5]
Increase throughput at little cost
Control is all within Analyst Software
Compliance not affected
Avoids contaminating MS inlet with early eluting compounds and solvent



Restrictions
Cannot do "Look Ahead"[6]
Injections are not staggered[7]
Peaks of interest must come off early
Columns may perform slightly different on alternating samples
Requires TrioVS valve
















Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-002 Dual Channel Serial Injection with Front Cut

Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste 		Analyst with ICC-CE * Trio VS
* Gradient Pump
* Regeneration pump 		* Saving of regeneration time(Back Cut)
* Increase throughput at little cost
* Control is all within Analyst
* Compliance is not affected
* Avoids contaminating MS inlet with early eluting compounds and Solvent 		* Can not do "Look Ahead"
* Injections are not staggered
* Peaks of interest must come of early
* Columns may perform slightly differently on alternating samples 		Yes

For other cost/time saving measures consider:

  • LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [8]
  • Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [9]
  • Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [10]
  • “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [11]
  • Dual Channel Parallel Injection (M-005) using LEAP Shell software [12]
  • Summary of LC Multi Valve Applications [13]
  • LEAP Shell Software and application for custom injections and scheduling [14]
leaptec.comleapwiki.com
leaptec.com
leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information

Contact the LEAP office

Retrieved from "http://www.leapwiki.com/mediawiki/index.php/M-002_Dual_Channel_Serial_Injection_with_front_cut_-_Analyst_with_ICC-CE"
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