M-002 Dual Channel Serial Injection with front cut - Analyst with ICC-CE
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|description = High throughput where one column is regenerating while the other is performing analytical separation. An additional valve allows solvent front to be cut to waste. | |description = High throughput where one column is regenerating while the other is performing analytical separation. An additional valve allows solvent front to be cut to waste. | ||
}} | }} | ||
+ | [[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]] | ||
+ | === Overview === | ||
+ | This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within '''Analyst Software'''[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=600927&tab=DetailInfo] from ''Applied Biosystems Inc'' using a Standard LEAP Cycle (requires ICC-CE software) [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-software/instrument-control-components-and-cycle-editor-181.php]. | ||
+ | There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/6-port-2-position-valve-155.php] for sample loading and a 10 port valve [http://www.leaptec.com/products/lc-related-instruments-and-accessories/ctc-lc-pal-injection-valves/10-port-2-position-valve-157.php]for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle. | ||
- | + | //[[Image:M-001.png|frame|Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system]] | |
- | + | <br><br><br><br> | |
+ | {| border="1" | ||
+ | | ''' Key Benefits ''' | ||
+ | |} | ||
+ | {| | ||
+ | |- | ||
+ | | Saving of Reagent time (Back cut) | ||
+ | |- | ||
+ | | Increase throughput at little cost | ||
+ | |- | ||
+ | | Control is all within Analyst Software | ||
+ | |- | ||
+ | | Compliance not affected | ||
+ | |- | ||
+ | | GLP Compliant | ||
+ | |} | ||
+ | <br><br> | ||
+ | {| border="1" | ||
+ | | '''Restrictions''' | ||
+ | |} | ||
+ | {| | ||
+ | |- | ||
+ | | Cannot do "Look Ahead" | ||
+ | |- | ||
+ | | Injections are not staggered | ||
+ | |- | ||
+ | | Peaks of interest must come off early | ||
+ | |- | ||
+ | | Columns may perform slightly different on alternating samples | ||
+ | |- | ||
+ | |} | ||
+ | <br><br><br><br><br><br> | ||
+ | |||
+ | |||
+ | {| cellpadding="6" style="border:1px solid darkgray;" | ||
+ | |- align=left | ||
+ | !width="20"|Code | ||
+ | !width="195"|Application | ||
+ | !width="85"|Control SW | ||
+ | !width="140"|Key Hardware | ||
+ | !width="205"|Key Benefits | ||
+ | !width="235"|Restrictions | ||
+ | !width="15"|GLP | ||
+ | |- align=left | ||
+ | | style="border:1px solid blue;"|'''M-002''' | ||
+ | | style="border:1px solid blue;"|'''Dual Channel Serial Injection with Front Cut'''<br><br>Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste | ||
+ | | style="border:1px solid blue;"|Analyst with ICC-CE | ||
+ | | style="border:1px solid blue;"|* Trio VS* Gradient Pump<br>* Regeneration pump | ||
+ | | style="border:1px solid blue;"|* Saving of regeneration time(Back Cut)<br>* Increase throughput at little cost<br> * Control is all within Analyst<br>* Compliance is not affected <br>* Avoids contaminating MS inlet with early eluting compounds and colvent | ||
+ | | style="border:1px solid blue;"|* Can not do "Look Ahead"<br>* Injections are not staggered<br>* Peaks of interest must come of early<br>* Columns may perform slightly differently on alternating samples | ||
+ | | style="border:1px solid blue;"|Yes | ||
+ | |} | ||
+ | |||
+ | |||
+ | === For other cost/time saving measures consider:=== | ||
+ | * LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing] | ||
+ | * Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-002_Dual_Channel_Serial_Injection_with_front_cut_-_Analyst_with_ICC-CE] | ||
+ | * Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [http://www.leapwiki.com/mediawiki/index.php?title=M-003_Dual_Channel_Parallel_Injection_-_Cycle_Composer] | ||
+ | * “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [http://www.leaptec.com/software/leap-shell-3.php] | ||
+ | * Dual Channel Parallel Injection (M-005) using LEAP Shell software [http://www.leapwiki.com/mediawiki/index.php?title=M-005_Dual_Channel_Parallel_Injection_-_LEAP_Shell_%22(M-Fab)%22] | ||
+ | * LEAP Shell Software and application for custom injections and scheduling [http://www.leaptec.com/software/leap-shell-3.php] | ||
---- | ---- | ||
+ | [[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]] | ||
+ | |||
=== Contact LEAP === | === Contact LEAP === | ||
+ | For additional information about this technique please contact LEAP Technologies for detailed information | ||
* [http://www.leaptec.com/contact-us.php Contact the LEAP office] | * [http://www.leaptec.com/contact-us.php Contact the LEAP office] |
Revision as of 21:32, 28 April 2009
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[3] for sample loading and a 10 port valve [4]for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.
//
Key Benefits |
Saving of Reagent time (Back cut) |
Increase throughput at little cost |
Control is all within Analyst Software |
Compliance not affected |
GLP Compliant |
Restrictions |
Cannot do "Look Ahead" |
Injections are not staggered |
Peaks of interest must come off early |
Columns may perform slightly different on alternating samples |
Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
---|---|---|---|---|---|---|
M-002 | Dual Channel Serial Injection with Front Cut Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste | Analyst with ICC-CE | * Trio VS* Gradient Pump * Regeneration pump | * Saving of regeneration time(Back Cut) * Increase throughput at little cost * Control is all within Analyst * Compliance is not affected * Avoids contaminating MS inlet with early eluting compounds and colvent | * Can not do "Look Ahead" * Injections are not staggered * Peaks of interest must come of early * Columns may perform slightly differently on alternating samples | Yes |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [5]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [6]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [7]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [8]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [9]
- LEAP Shell Software and application for custom injections and scheduling [10]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information