M-001 Dual Channel Serial Injection - Analyst with ICC-CE
From LEAP
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- | + | !width="25"|Code | |
- | ! | + | !width="200"|Application |
- | |- | + | !width="90"|Control SW |
- | + | !width="200"|Key Hardware | |
- | | | + | !width="200"|Key Benefits |
+ | !width="200"|Restrictions | ||
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+ | | style="border:1px solid blue;"|'''M-001''' | ||
+ | | style="border:1px solid blue;"|'''Column switching with parallel regeneration'''<br><br>Provides shorter cycle times by regenerating columns during next analytical run | ||
+ | | style="border:1px solid blue;"|Analyst with ICC-CE | ||
+ | | style="border:1px solid blue;"|* 6 port injection valve<BR>* 10 port Valve <br>* Gradient Pump<br>* Regeneration pump | ||
+ | | style="border:1px solid blue;"|* Saving of regeneration time(Back Cut)<br>* Increase throughput at little cost<br> * Control is all within Analyst<br>* Compliance is not affected | ||
+ | | style="border:1px solid blue;"|* Can not do "Look Ahead"<br>* Injections are not staggered<br>* Peaks of interest must come of early<br>* Columns may perform slightly differently on alternating samples | ||
+ | | style="border:1px solid blue;"|Yes | ||
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=== PRINT Function for Page === | === PRINT Function for Page === |
Revision as of 21:00, 28 April 2009
LC Column Switching & Regeneration | |
Application Type | |
SPECIAL | |
Application ID | |
M-001 | |
Description | |
LC Column Switching & Regeneration. |
Contents |
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[3] for sample loading and a 10 port valve [4]for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.
Key Benefits |
Saving of Reagent time (Back cut) |
Increase throughput at little cost |
Control is all within Analyst Software |
Compliance not affected |
GLP Compliant |
Restrictions |
Cannot do "Look Ahead" |
Injections are not staggered |
Peaks of interest must come off early |
Columns may perform slightly different on alternating samples |
Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
---|---|---|---|---|---|---|
M-001 | Column switching with parallel regeneration Provides shorter cycle times by regenerating columns during next analytical run | Analyst with ICC-CE | * 6 port injection valve * 10 port Valve * Gradient Pump * Regeneration pump | * Saving of regeneration time(Back Cut) * Increase throughput at little cost * Control is all within Analyst * Compliance is not affected | * Can not do "Look Ahead" * Injections are not staggered * Peaks of interest must come of early * Columns may perform slightly differently on alternating samples | Yes |
PRINT Function for Page
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [5]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [6]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [7]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [8]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [9]
- LEAP Shell Software and application for custom injections and scheduling [10]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information