M-001 Dual Channel Serial Injection - Analyst with ICC-CE
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| - | ! M-001 !! Column | + | ! M-001 !! Column switching with parallel regeneration <br>Provides shorter cycle times by regenerating columns during next analytical run!! Analyst with ICC-CE !! PAL<BR>6 port injection valve<BR>10 port Valve <br>Gradient Pump and Regeneration pump!! Saving of regeneration time(Back Cut)<br> Increase throughput at little cost<br> Control is all within Analyst<br> Compliance is not affected !! Can not do "Look Ahead"<br> Injections are not staggered<br> Peaks of interest must come of early<br>Columns may perform slightly differently on alternating samples!! Yes |
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Revision as of 20:09, 28 April 2009
| LC Column Switching & Regeneration |
| Application Type | |
| SPECIAL | |
| Application ID | |
| M-001 | |
| Description | |
| LC Column Switching & Regeneration. |
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Contents |
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst Software[1] from Applied Biosystems Inc using a Standard LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration. This configuration requires a 6 port valve[3] for sample loading and a 10 port valve [4]for column switching. Both valves must be controlled by the Standard LEAP cycle. LEAP can not control the MS Selector valve as part of the cycle.
Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system
| Key Benefits |
| Saving of Reagent time (Back cut) |
| Increase throughput at little cost |
| Control is all within Analyst Software |
| Compliance not affected |
| GLP Compliant |
| Restrictions |
| Cannot do "Look Ahead" |
| Injections are not staggered |
| Peaks of interest must come off early |
| Columns may perform slightly different on alternating samples |
| Quick Overview | ||||||
| Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
|---|---|---|---|---|---|---|
| M-001 | Column switching with parallel regeneration Provides shorter cycle times by regenerating columns during next analytical run | Analyst with ICC-CE | PAL 6 port injection valve 10 port Valve Gradient Pump and Regeneration pump | Saving of regeneration time(Back Cut) Increase throughput at little cost Control is all within Analyst Compliance is not affected | Can not do "Look Ahead" Injections are not staggered Peaks of interest must come of early Columns may perform slightly differently on alternating samples | Yes |
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For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [5]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [6]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [7]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [8]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [9]
- LEAP Shell Software and application for custom injections and scheduling [10]
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Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
