LC Multi-Valve Applications

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Revision as of 16:20, 7 October 2009

LC Multiple Valve Applications
Application Type
  STANDARD AND SPECIAL
Application ID
 
Description
  Multiple Valve Applications for LC

Overview

This summary is a sampling list of what LEAP has done and can do for you. All of these are focused on getting you Higher Throughput


Specific Categories

There are many techniques and applications that LEAP can help you with to increase sample throughput with automated injection HPLC systems. From "Front Cuts" to "Heart Cuts" to "Back Cuts" to "Multiplexing". Typical applications are pre-column SPE, pre-column cleanup, sample desalting,parallel column regeneration, etc. LEAP has the experience to solve your throughput needs. These are just a few of our offerings. We integrate multiple vendors HPLC systems and have a wide array of injection valves and selector valves to get you the best result. Many of these can be added to your existing systems to better utilize the hardware you already have.


Videos
Movie of the HTS PAL injecting sample in valve and washing syringe.


Print
PDF of Summary LC Multi Valve_Applications page formatted for printing



Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-001
[1]
Dual Channel Serial Injection

Provides shorter cycle times by regenerating columns during next analytical run
Analyst with ICC-CE * 6 port injection valve
* 10 port Valve
* Gradient Pump
* Regeneration pump
* Saving of regeneration time(Back Cut)
* Increase throughput at little cost
* Control is all within Analyst
* Compliance is not affected
* Can not do "Look Ahead"
* Injections are not staggered
* Peaks of interest must come of early
* Columns may perform slightly differently on alternating samples
Yes
Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-002
[2]
Dual Channel Serial Injection with Front Cut

Provides shorter cycle times by regenerating columns during next analytical run and diverts the solvent front to waste
Analyst with ICC-CE * Trio VS
* Gradient Pump
* Regeneration pump
* Saving of regeneration time(Back Cut)
* Increase throughput at little cost
* Control is all within Analyst
* Compliance is not affected
* Avoids contaminating MS inlet with early eluting compounds and solvent
* Can not do "Look Ahead"
* Injections are not staggered
* Peaks of interest must come of early
* Columns may perform slightly differently on alternating samples
Yes


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-003
[3]
Dual Channel Parallel Injection

Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition
Cycle Composer outside of Analyst * DoubleStack valve
* 6 port injection valve
* FWS in front of 6 port valve
* Gradient Pump
* Regeneration pump
* Saving of Front and Back Cut
* Provides "Look Ahead"
* Provides staggering of injections
* Requires matching sample lists in Cycle Composer and Analyst
* Columns may perform slightly differently on alternating samples
No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-004
[4]
Analyst with "Look Ahead" with LEAP Shell

Control of the Sample List is through LEAP Shell
LEAP Shell * Standard PAL
* Pumps are supported within Analyst
* "Look Ahead"
* Can import custom columns
* Freedom to create and use custom Cycles
* Does not support 21CFR11 compliance
No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-005
[5]
Dual Channel Parallel Injection

Provides minimum cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition
LEAP Shell * DoubleStack valve
* 6 port injection valve
* FWS in front of 6 port valve
* Gradient Pump
* Regeneration pump
* Integration with Analyst Software
* Single Sample List
* Provides staggering of injections
* Multiplexes 2 separate LC systems into on MS
* Does not support 21CFR11 compliance
* Requires pumps to be integrated with LEAP Shell
No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-006
[6]
Inline Sample Concentration

SPE Column regenerated during analytical extraction
Analyst with ICC-CE * TrioVS
* Automated concentration and elution of sample on SPE column prior to separation on analytical column * No "Look Ahead"
* Can not prep samples ahead of analytical run
No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-007
[7]
Inline Sample Filtration

SPE Column regenerated during analytical extraction
Analyst with ICC-CE * TrioVS
* Automated filtration of sample on cleanup column prior to separation on analytical column * No "Look Ahead"
* Can not prep samples ahead of analytical run
No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-008
[8]
Dual Channel In-line Sample Concentration

SPE Column regenerated during analytical extraction
Cycle Composer outside of Analyst * TrioVS
* Dual Stream
* High throughput sample cleanup "Look Ahead"
* Requires two matching sample lists No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-009
[9]
Dual Channel in-line Sample Filtration

SPE Column regenerated during analytical extraction
Cycle Composer outside of Analyst * TrioVS
* Dual Stream
* High throughput sample cleanup
* "Look Ahead"
* Requires two matching sample lists No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-010
[10]
Dual Channel in-line Sample Concentration

SPE Column regenerated during analytical extraction
LEAP Shell * TrioVS
* High throughput sample cleanup "Look Ahead" No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-011
[11]
Dual Channel in-line Sample Filtration

SPE Column regenerated during analytical extraction
LEAP Shell * TrioVS
* High throughput sample cleanup "Look Ahead" No


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-014
[12]
Selectable HPLC

(max 2 Streams)
Analyst Standard Cycles * Two Injection Valves
* MS Stream Selector
* Different Analyst Methods inject into different LC Valves
* Uses Standard Analyst cycles
* Requires Analyst control of the stream selector valve
* Max of 2 streams can be supported
Yes


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-015
[13]
Selectable HPLC

(max 4 Streams)
Analyst with ICC-CE * Multiple Injection Valves (serial control)
* PAL 10 port Valve for Stream Selector
* Different Analyst Methods inject into different LC Valves
* Stream selection to MS
* PAL controls the stream selector valve
* Requires Custom Cycle
Yes


Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-016
[14]
Column Selection

Method selects which HPLC column is in-line before injection
Analyst with ICC-CE * CTC Column Selector
* Up to 5 columns
* Automated switching between analytical columns within run * Requires Custom Cycles Yes


Contact LEAP

Contact the LEAP office

Keywords: Dual Channel, Single channel, Serial Injection, shorter cycle times, regeneration of columns, PAL, 10 port valve, gradient pump, regeneration pump, 6 port injection valve, saving, back cut, increase throughput, compliance, Look Ahead, staggered injections, Peaks of interest early, columns, alternating samples, front cut, divert to waste, solvent, TRIO VS, saving of regeneration back cut, parallel injections, staggering of injections, minimum cycle times, acquisition time, Flux pumps, Multiplexes, 2 separate LC systems into one Mass Spec, 21CFR11 compliance, In-line sample concentration, SPE Column regenerated during analytical extraction, prep samples ahead of analytical run, In line sample filtration, In-line concentration, Dual Stream, sample cleanup, column regeneration, concentration, Analyst Cycles, Stream Selector valve, PAL control, custom cycles, macros, CTC Column selector, Column selection, 4 streams, selectable HPLC, 2 streams, 4 streams, Analyst Software, ICC-CE software,LEAP Shell software,cycle composer software

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