Balance PAL

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(New page: {{LSApp |name = Balance PAL |image = PlantExtract1.png |type = Sample Prep and Inject |id = Balance PAL |description = System for integrating a Balan...)
Current revision (00:37, 21 October 2010) (view source)
 
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{{LSApp
{{LSApp
   |name        = Balance PAL
   |name        = Balance PAL
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   |image      = PlantExtract1.png
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   |image      = Balance_HTS.png
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   |type        = Sample Prep and Inject
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   |type        = Sample Prep
   |id          = Balance PAL
   |id          = Balance PAL
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   |description = System for integrating a Balance with the PAL
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   |description = Robotic Automated System for integrating a Balance with the PAL Robot
}}
}}
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{{logo}}
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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[[Image:Automated_Balance.png|600px|thumb|center|LEAP CTC Robotic Weighing Station ]]
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'''Automated Weighting Station'''
=== Overview ===
=== Overview ===
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Plant material and biomass in general are not just for food and feed any longer. They have become the feedstock for fuel, neutraceuticals, drugs and food supplements such as OMEGA-3 or other strong antioxidants. There is a growing research community that looks from a genetics and maximum yield perspective at many bio-organism. A primary example are seeds for oil or algae for OMEGA-3. All these examples have one complication in common: Before analysis for the presence of certain chemicals (molecules) an extract has to be generated out of the raw sample. Fibers, skin and other natural tissue has to be filtered and many constituants of the plants that are of interest have to be extracted in an extraction and separation process. There are many procedures that use a manual approach. As long as the methodology of plant research is its trial and error stage automation can't be justified. However, once a systematic work flow is well defined an automated process is not only more productive but leads to more consistency and less wasteful use of materials, disposables, solvents and of course highly educated personnel's time.
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* Automate simple tedious weighing repetitions for liquids
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LEAP had many customers that successfully got their extractions automated. Some started out with Seeds, Algae, Tobacco and other leafy plants. The automation was in many cases "just-in time" sample preparation on LEAP's primary XYZ platform the CTC PAL with a subsequent injection onto a Gas Chromatograph or Mass Spectrometer.
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* Tare weight, fill weight, solvent volume calculation in spreadsheet
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* Reagent additions
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* Accurate dilutions
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* Monitoring of weight change reactions (outgassing,digestions)
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* Vial transportation
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* Two-way communication between Balance and PAL
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* Most cost effective; low price tag
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* Control it with LEAP Shell
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* Combines liquid transfers with gravimetric determinations
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* Adaptable time schedule
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* Adaptable to various vial sizes (2, 5, 10 or 20ml vials)
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* Results from weighing recorded in LEAP Shell software
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* Optional automatic bar code scanner
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* Small footprint
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* High vial capacity
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* Many PAL accessories
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Often a GC Twin PAL accomplishes the extraction and filtration before a GC analyzes plant extract material. The application is to take a vial containing plant material, add solvent, heat & agitator, filter, and then analyze on a 6890 GC.  This application may be applicable to any customer who wants to do filtration providing the liquid can be pushed thru the filter plate with a low pressure inert gas through the headspace syringe.
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=== Sample Balance PAL Workflow ===
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* Tare bar coded empty vials
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* Manually add powder solids w/ measuring spoon
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OR Automatically add liquid samples with PAL
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* Weigh barcoded vials
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* Calculate solvent needed for target concentration
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* Dispense solvent
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* Agitate with optional temperature control
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* Inject onto HPLC valve
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OR manually remove vials for off-line analysis
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Additional accessories such as an integrated balance (Mettler) and a barcode scanner[http://leapwiki.com/mediawiki/index.php?title=Bar_Code_Reading] have been added in the past.
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=== Photos ===
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<gallery>
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  Image:Balance_HTS.png|LEAP CTC Balance PAL
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  Image:Balance_HTS_Top_view.png|LEAP CTC Balance PAL Top View
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  Image:Automated_Balance.png|LEAP CTC Automated Weighing Station Overview
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  Image:Balance_with_gripper_and_open_20_ml_vial.jpg |LEAP Gripper with 20 ml open vial
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</gallery>
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'''Markets Served:'''<br>
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=== Other Reference Material ===
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Elements of this application can be applied to labs requiring any type of filtration of a sample while maximizing throughput.
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[[image:Download Icon.png|40px]]  [[:Media:Balance_PAL.pdf| LEAP Weighing Station Information Document]]<br>
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* University
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* Research
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* FDA
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* EPA
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* USDA
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=== Accessories for the PAL ===
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[[image:Accessories Icon.png|40px]] [http://leapwiki.com/mediawiki/index.php?title=Bar_Code_Reading Bar Code Reading]
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[[image:Accessories Icon.png|40px]] [http://leapwiki.com/mediawiki/index.php?title=Cooling_and_Heating_Sample_Storage Cooling &Heating Sample Storage]
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'''What is a Plant Extract experiment?'''<br/>
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[[image:Accessories Icon.png|40px]] [http://leapwiki.com/mediawiki/index.php?title=Large_Volume_Dispensing Large Volume Dispensing]
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The sample prep procedure is quite simple with the exception of the final sample needing to not contain any of the plant material when it gets to the analysis procedure. The samples are incubated in a heated agitator, and then allowed to settle.  The sample solvent, possible containing small pieces of plant material, is injected onto a 96 well filter plate in the top of a SPE drawer with a 19ga headspace syringe using the Combi PAL, the needle guide is locked, and then flushed (purged) with a stream of inert gas (e.g. Helium) forcing the solvent through the filter plate into the receiving plate below. The other PAL then makes the liquid injection from the receiving plate into the GC.
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[[image:Accessories Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Shaking%2C_Heating_of_Samples Mixing and Heating of Samples]
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'''LEAPS Approach'''<br/>
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[[image:Accessories Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Category:Accessories Other Accessories for PAL Robots]
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The GC Twin PAL in combination with the cycle composer makes a very effective and flexible workstation. We were able to design and provide a solution which met the following challenges from the customer:<br/>
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# Provide the necessary filtration to have clean samples to analyze. In order to get good blow-out of the liquid through the filter, it was determined that the filter plate should be sealed and have a small nozzle on the underside to ensure accurate collection without crossover to adjacent collection vials.<br/>
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# Throughput. By using a Twin PAL we are able to take advantage of having one PAL do the preparation while the other does the sample injections.<br/>
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# Spatial concerns. Provide a way to get everything that was required to fit. <br/>
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<br/>
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=== Photos ===
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=== Videos of the PAL ===
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<br>
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[[Image:Movie Icon.png|40px]]
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[http://www.youtube.com/user/LEAPTechnologies#g/u LEAP's PAL Application Videos on YouTube]
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<br>
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----
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{{logo}}
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<gallery>
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[http://www.leaptec.com/ LEAP Technologies] provides fully automated sample processing workstation instrumentation solutions based on the LEAP CTC [http://leapwiki.com/mediawiki/index.php?title=Category:PAL PAL] X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP also offers process control systems and stand alone workstations. LEAP is an open, neutral resource for technical support of existing PAL. Keep your PAL in peak working order LEAP offers full [http://www.leapwiki.com/mediawiki/index.php?title=Category:Service support and service] for the PAL platform with over 11 years of history with the PAL. LEAP can also write custom macros, [http://www.leapwiki.com/mediawiki/index.php?title=Category:Cycles cycles], and scheduling [http://www.leapwiki.com/mediawiki/index.php?title=Category:Software software] for your specific applications. We're proud that LEAP is known as a productivity improver for laboratories including government labs in agencies such as FDA, USDA, EPA, FBI, Armed Forces, Homeland Security and others function more efficiently. Robotic sample prep equipment works well in government labs and challenges lab technicians to keep feeding those more samples. Analytical equipment such as chromatographs and mass spectrometers are expensive devices and also expensive to maintain. LEAP can help to keep them running and analyze samples around the clock. LEAP has most of its laboratory automation robots under [http://leapwiki.com/mediawiki/index.php?title=Category:GSA GSA]contract and is eager to service and maintain everything it sells to keep your expensive lab equipment running around the clock. CTC Analytics Combi PAL and HTS PAL for Front-End Automation for LC and GC applications. LEAP offers customized on-site training. LEAP Is continuing to develop FAQ, Solutions, Tips & Techniques,a Learning Center with the LEAP Wiki for the benefit of all PAL Platform Robotic users. We will continue exceeding customer expectations for quality, cost effective and efficient results using the most current technology and expertise in our industry. Our aim is to be the ultimate knowledge base on everything related to the PAL. LEAP is known as the autosamplerguys being in business over twenty years. LEAP has extensive multi-vendor experience with integrating the PAL. HTX, HTS, HTC, HTX-xt, HTS-xt, HTC-xt, LC-Mini, Combi PAL,COMBI-xt PAL, and GC PAL models. We have the Quality, Training, Parts availability, Infrastructure, and Experience.LEAP is sometimes referred to as an Application Automation House who is continually adding new products and solutions to its Lab Automation line. As we do so we change the content of this web site so please check back.<br>Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions whether it is our standard applications or customized to your needs. The Combi-xt PAL autosampler is able to perform liquid and headspace analysis. Headspace options include Solid Phase Micro-extraction (SPME) and ITEX-2 as alternative to purge and trap. The HTS-xt PAL autosampler dramatically increases sample throughput by offering automated sample prep, Peltier cooled and heated sample storage using the cooled stack, Peltier cooled and heated sample storage using the cooled stack and low carryover due to the self-cleaning DLW (Dynamic Load and Wash) module and L-Mark syringes, peltier cooled valves and columns.  LEAP provides automation that fits your needs and reduces errors, increases sample capacity, increases efficiency, reduces cost by supplying long term productivity improvements with user friendly software and flexible hardware. LEAP performs just in time or on time sample prep and inject. LEAP offers full support of integrated and non-integrated PAL software with both sales and support for Cruise Control, Cycle Composer and Cycle Composer Macro Editor, Agilent ChemStation CTC Control, Mass Hunter and EZChrom CTC integration, Xcalibur, Waters Masslynx or Analyst CTC Control software, Thermo (Finnegan) XCalibur, LEAP Shell, and Chronos software. The PAL is more than just an autosampler, it is a high-quality and efficient laboratory robot platform that can be customized by LEAP Technologies to meet your specific needs.
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  Image:PlantExtract1.png| Automation of Plant Extract before Gas Chromatography
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  Image:Seed Crushing.png| Seed Crusher
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</gallery>
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=== Other Reference Material ===
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<gallery>
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image:The_Plant_Journal_(2006)_45,_847-856.pdf‎| A high-throughput screen for genes from castor that boost
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hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis 
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</gallery>
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[[Image:LEAP Logo.png|frame|[http://www.leaptec.com leaptec.com]<br />[http://www.leapwiki.com leapwiki.com]]]
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=== Contact LEAP ===
=== Contact LEAP ===
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{{contact|topic=LEAP and the PAL Platform}}
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<br>''The information and references contained in this web site are believed to be accurate and the most current information available.''
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For additional information about this technique please contact LEAP Technologies for detailed information
 
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* [http://www.leaptec.com/contact-us.php Contact the LEAP office]
 
[[Category:LEAP_Shell]]
[[Category:LEAP_Shell]]
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[[Category:Accessories]]
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[[Category:Application_Solutions]]
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[[Category:Glossary]]

Current revision

Balance PAL
Application Type
  Sample Prep
Application ID
  Balance PAL
Description
  Robotic Automated System for integrating a Balance with the PAL Robot
 ↳ leaptec.com  ↳ leapwiki.com
LEAP CTC Robotic Weighing Station
LEAP CTC Robotic Weighing Station

Automated Weighting Station

Contents

Overview

  • Automate simple tedious weighing repetitions for liquids
  • Tare weight, fill weight, solvent volume calculation in spreadsheet
  • Reagent additions
  • Accurate dilutions
  • Monitoring of weight change reactions (outgassing,digestions)
  • Vial transportation
  • Two-way communication between Balance and PAL
  • Most cost effective; low price tag
  • Control it with LEAP Shell
  • Combines liquid transfers with gravimetric determinations
  • Adaptable time schedule
  • Adaptable to various vial sizes (2, 5, 10 or 20ml vials)
  • Results from weighing recorded in LEAP Shell software
  • Optional automatic bar code scanner
  • Small footprint
  • High vial capacity
  • Many PAL accessories

Sample Balance PAL Workflow

  • Tare bar coded empty vials
  • Manually add powder solids w/ measuring spoon

OR Automatically add liquid samples with PAL

  • Weigh barcoded vials
  • Calculate solvent needed for target concentration
  • Dispense solvent
  • Agitate with optional temperature control
  • Inject onto HPLC valve

OR manually remove vials for off-line analysis

Photos

Other Reference Material

LEAP Weighing Station Information Document

Accessories for the PAL

Bar Code Reading

Cooling &Heating Sample Storage

Large Volume Dispensing

Mixing and Heating of Samples

Other Accessories for PAL Robots

Videos of the PAL


LEAP's PAL Application Videos on YouTube
-


 ↳ leaptec.com  ↳ leapwiki.com

LEAP Technologies provides fully automated sample processing workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography. LEAP also offers process control systems and stand alone workstations. LEAP is an open, neutral resource for technical support of existing PAL. Keep your PAL in peak working order LEAP offers full support and service for the PAL platform with over 11 years of history with the PAL. LEAP can also write custom macros, cycles, and scheduling software for your specific applications. We're proud that LEAP is known as a productivity improver for laboratories including government labs in agencies such as FDA, USDA, EPA, FBI, Armed Forces, Homeland Security and others function more efficiently. Robotic sample prep equipment works well in government labs and challenges lab technicians to keep feeding those more samples. Analytical equipment such as chromatographs and mass spectrometers are expensive devices and also expensive to maintain. LEAP can help to keep them running and analyze samples around the clock. LEAP has most of its laboratory automation robots under GSAcontract and is eager to service and maintain everything it sells to keep your expensive lab equipment running around the clock. CTC Analytics Combi PAL and HTS PAL for Front-End Automation for LC and GC applications. LEAP offers customized on-site training. LEAP Is continuing to develop FAQ, Solutions, Tips & Techniques,a Learning Center with the LEAP Wiki for the benefit of all PAL Platform Robotic users. We will continue exceeding customer expectations for quality, cost effective and efficient results using the most current technology and expertise in our industry. Our aim is to be the ultimate knowledge base on everything related to the PAL. LEAP is known as the autosamplerguys being in business over twenty years. LEAP has extensive multi-vendor experience with integrating the PAL. HTX, HTS, HTC, HTX-xt, HTS-xt, HTC-xt, LC-Mini, Combi PAL,COMBI-xt PAL, and GC PAL models. We have the Quality, Training, Parts availability, Infrastructure, and Experience.LEAP is sometimes referred to as an Application Automation House who is continually adding new products and solutions to its Lab Automation line. As we do so we change the content of this web site so please check back.
Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions whether it is our standard applications or customized to your needs. The Combi-xt PAL autosampler is able to perform liquid and headspace analysis. Headspace options include Solid Phase Micro-extraction (SPME) and ITEX-2 as alternative to purge and trap. The HTS-xt PAL autosampler dramatically increases sample throughput by offering automated sample prep, Peltier cooled and heated sample storage using the cooled stack, Peltier cooled and heated sample storage using the cooled stack and low carryover due to the self-cleaning DLW (Dynamic Load and Wash) module and L-Mark syringes, peltier cooled valves and columns. LEAP provides automation that fits your needs and reduces errors, increases sample capacity, increases efficiency, reduces cost by supplying long term productivity improvements with user friendly software and flexible hardware. LEAP performs just in time or on time sample prep and inject. LEAP offers full support of integrated and non-integrated PAL software with both sales and support for Cruise Control, Cycle Composer and Cycle Composer Macro Editor, Agilent ChemStation CTC Control, Mass Hunter and EZChrom CTC integration, Xcalibur, Waters Masslynx or Analyst CTC Control software, Thermo (Finnegan) XCalibur, LEAP Shell, and Chronos software. The PAL is more than just an autosampler, it is a high-quality and efficient laboratory robot platform that can be customized by LEAP Technologies to meet your specific needs.

Contact LEAP

Contact LEAP

For additional information about LEAP and the PAL Platform, please contact LEAP Technologies.


The information and references contained in this web site are believed to be accurate and the most current information available.

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