M-001 Dual Channel Serial Injection - Analyst with ICC-CE
From LEAP
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''Please note: LEAP can not control the MS Selector valve as part of the cycle.'' | ''Please note: LEAP can not control the MS Selector valve as part of the cycle.'' | ||
- | [[Image:M- | + | [[Image:M-001w.png|frame|Plumbing of valve diagram showing flow for each selected column with the regeneration of the analytical column while the other is engaged with the LCMS system]] |
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=== Typical System of PAL with CoolStack Fast Wash and Valves === | === Typical System of PAL with CoolStack Fast Wash and Valves === | ||
[[Image:M-001_HTC_CoolStack.png|Typical system for Dual Column with Regeneration]] | [[Image:M-001_HTC_CoolStack.png|Typical system for Dual Column with Regeneration]] | ||
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=== PRINT Function for Page === | === PRINT Function for Page === |
Revision as of 20:54, 13 May 2009
LC Column Switching & Regeneration | |
Application Type | |
SPECIAL | |
Application ID | |
M-001 | |
Description | |
LC Column Switching & Regeneration. |
Contents |
Overview
This configuration allows the regeneration of one of the analytical columns while the other one is engaged - giving greater efficiency and higher throughput. This is run directly from within Analyst® Software[1] from Applied Biosystems Inc® using a LEAP Cycle (requires ICC-CE software) [2].
There are 2 pumps needed, one for the gradient run and another (higher flow rate and isocratic) for regeneration [3]. This configuration requires a 6 port valve[4] for sample loading and a 10 port valve [5]for column switching. Both valves must be controlled by the LEAP cycle.
Please note: LEAP can not control the MS Selector valve as part of the cycle.
Key Benefits |
Saving of Reagent time (Back cut)[6] |
Increase throughput at little cost |
Control is all within Analyst Software |
Compliance not affected |
GLP Compliant |
Restrictions |
Cannot do "Look Ahead"[7] |
Injections are not staggered[8] |
Peaks of interest must come off early |
Columns may perform slightly different on alternating samples |
Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
---|---|---|---|---|---|---|
M-001 | Dual Channel Serial Injection Provides shorter cycle times by regenerating columns during next analytical run | Analyst with ICC-CE | * 6 port injection valve * 10 port Valve * Gradient Pump * Regeneration pump | * Saving of regeneration time(Back Cut) * Increase throughput at little cost * Control is all within Analyst * Compliance is not affected | * Can not do "Look Ahead" * Injections are not staggered * Peaks of interest must come of early * Columns may perform slightly differently on alternating samples | Yes |
Typical System of PAL with CoolStack Fast Wash and Valves
PRINT Function for Page
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [9]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [10]
- Dual Channel Parallel Injection (M-003) using Cycle Composer Software and Analyst [11]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [12]
- Dual Channel Parallel Injection (M-005) using LEAP Shell software [13]
- LEAP Shell Software and application for custom injections and scheduling [14]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information