Small Volume Fraction Collection

From LEAP

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
{{LSApp
{{LSApp
-
   |name        = Purific
+
   |name        = Collect PAL
-
   |image      = Mini.png
+
   |image      = Collect1.png
   |type        = '''SPECIAL'''
   |type        = '''SPECIAL'''
   |id          =  
   |id          =  
-
   |description = Purific
+
   |description = Collect PAL
}}
}}
=== Overview ===
=== Overview ===
-
PEAK HARVESTER is a software package used for the preparation and realization of fractionation that brings together components from different manufacturers to create an integrated system. The collector's unit, the pumps, detectors, and the data acquisition are implemented as individual systems within a single software package. The central component is the PAL robot and all other devices can be either directly controlled by the PEAK HARVESTER or synchronized with injection and collection by contact closure and other analog.
+
The Collect PAL as shown can hold up to 24-shallow well plates and collect into either 96-well or 384-well plates.  It is shown with its waste drain and high pressure valve set up.  With this valve it is able to use down to 0.005” id PEEK tubing.
-
The Purific combines LEAP’s strengths in LC sample preparation and purification to enhance productivity for analytical chemist.
+
Compact in size, the Collect PAL covers a bench area of only 28”x28” and has a height of only 24” to the top of its Z-arm.
 +
The following slides display a variety of standard configurations and describe the working specifications under those configurations.
 +
The slide show ends with a brief discussion of the LEAP Shell Software package and a couple of screen shots to indicate the user-interface.
-
* Dual head minimizes carryover and increases throughput
+
Key Features –
-
* Syringe only concept with no error prone Teflon tubing or transfer line
+
* Zero Loss Collection – most fraction collectors either are a drop-type or a solenoid driven-type. 
-
* Precise sample handling for dependable purification results
+
* Zero Drips, Zero Spills
-
* All in one Injector-Collector, Compact Ergonomic Design features a small footprint that fits easily into a standard fume hood
+
* The drop type fraction collector simply flows the effluent through tubing to a movable arm and positions the tubing over a well or tube and stays in that position for a specified time until moving to the next collection site.  The main concern with this type of fraction collection is the complete lack of control over volume in the fraction site and, more importantly, the placement of each and every drop in a tube.  There should be concern that sample can be lost during the “in-between” of sites during a move.
-
* Anti-Static solvent Lines
+
* The solenoid drive fraction collector solves the drop between sites problem by quickly switching a solenoid valve off/on during the move between wells. Drip solved, but now valuable sample during that movement is sent to a waste stream. Sample is lost to the waste.
-
* Automated switching procedures to clean and equilibrate column to increase throughput. Requires optional 10 port valve.
+
*  Neither type of fraction collection option protects what is most important – the sample -- and provides a safe environment for the researcher.
-
* Performance with Maximum Flexibility
+
-
* Keep your fractions cool, up to 4°C, with optional cool stack
+
-
 
+
-
 
+
-
 
+
-
[[Image:PEAK Harvester SW.png|250px|thumb|left|PEAK Harvester Software]]
+
-
 
+
-
=== Peak Harvester Control Software ===
+
-
Peak Harvester™ Developed by Leap Technologies is specifically designed to control each single step of the purification process. It allows control of the LC pumps and detector, collects fractions in manual mode or in a choice of three programmable modes inside a predetermined collection window:
+
-
 
+
-
* Time based mode using individual time slices per sample
+
-
* Peak-Based mode using an analog signal e.g. UV or ELSD detector
+
-
* Mass-triggered mode using an output signal of the MS for mass fractionation
+
-
* Color coded sample and fraction tracking lets you review and report an enormous amount of analytical data. It shows how many fractions have been collected and where they are located.
+
-
* Advanced software algorithms allow collecting the fractions correctly even for challenging peak shapes.
+
-
 
+
-
 
+
-
 
+
-
PeakHarvester™ software incorporates an open access interface that lets you walk up an log in your samples anytime. First simply log into the software using your ID followed by the format of the samples. Choose the method you want to use from a set of method options. After the collection process, review your results and retrieve the purified samples. PeakHarvester™ contains complete system administration tools for secure access management. All activities are stored in log files to ensure anytime system security reducing the possibility of errors and subsequent losses of potentially precious materials. PeakHarvester™ track barcode and accept sample list in different format like CSV or XML and interface easily with your LIMS systems with its XML report capability
+

Revision as of 00:45, 3 April 2009

Collect PAL
Application Type
  SPECIAL
Application ID
 
Description
  Collect PAL

Overview

The Collect PAL as shown can hold up to 24-shallow well plates and collect into either 96-well or 384-well plates. It is shown with its waste drain and high pressure valve set up. With this valve it is able to use down to 0.005” id PEEK tubing.

Compact in size, the Collect PAL covers a bench area of only 28”x28” and has a height of only 24” to the top of its Z-arm. The following slides display a variety of standard configurations and describe the working specifications under those configurations. The slide show ends with a brief discussion of the LEAP Shell Software package and a couple of screen shots to indicate the user-interface.

Key Features –

  • Zero Loss Collection – most fraction collectors either are a drop-type or a solenoid driven-type.
  • Zero Drips, Zero Spills
  • The drop type fraction collector simply flows the effluent through tubing to a movable arm and positions the tubing over a well or tube and stays in that position for a specified time until moving to the next collection site. The main concern with this type of fraction collection is the complete lack of control over volume in the fraction site and, more importantly, the placement of each and every drop in a tube. There should be concern that sample can be lost during the “in-between” of sites during a move.
  • The solenoid drive fraction collector solves the drop between sites problem by quickly switching a solenoid valve off/on during the move between wells. Drip solved, but now valuable sample during that movement is sent to a waste stream. Sample is lost to the waste.
  • Neither type of fraction collection option protects what is most important – the sample -- and provides a safe environment for the researcher.


Screenshots

Purific System

PEAL Harvester Software

Contact LEAP

Retrieved from "http://www.leapwiki.com/mediawiki/index.php/Small_Volume_Fraction_Collection"