MALDI Spotting with the PAL
From LEAP
| MALDI Spotting with the PAL |
| Application Type | |
| STANDARD | |
| Application ID | |
| MALDI Spotting | |
| Description | |
| MALDI Spotting with the PAL |
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Contents |
Abstract
Solid phase microextraction, or SPME, is a sample preparation technique used both in the laboratory and on-site. Developed in the early 1990s at the University of Waterloo by Dr. Pawliszyn's group, it is a simple and inexpensive technique where the use of solvents is not necessary.
Photos
 Sample vial tray |
 Magnetic tool tip |
 Bar code reader |
 Agitator |
 Agitator |
 Vial in transfer |
 Agilent 6890 GC / 5975C VL MSD |
 SPME Adapter and Fibers |
 CTC Autosampler PAL with SPME |
CTC Twin PAL Autosampler with Sample Prep and SPME |
 Needle Heater Optional Accessory for SPME |
 Needle Heater Optional Accessory for SPME |
SPME offers some important advantages:
- Single Step Extraction - reduces sample preparation time by 70%
- Versatility - various selectivities available, adapts to any GC or HPLC system, can be automated
- Efficient & Economical - little/no use of solvents, quantitative method, allows more than 50 extractions per fiber on average
Comparison of SPME to SPE
SPE exhaustively extracts all of the analytes, but they are diluted during elution 1mL of sample containing 1µg/mL of analyte passed through tube, eluted with 1mL of solvent = 1µg/mL X 1µl injection = 1ng (Concentrating to 100µl or injecting 10µl = 10ng on column
SPME partially extracts analytes but desorbs the entire amount 1mL sample containing 1µg/mL of analyte extracted with fiber. 1% goes on fiber. 0.01 x 1µg = 10ng desorbed on column
Useful links
www.leapwiki.com
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
