From LEAP
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| - | === SPME offers some important advantages: ===
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| - | * Single Step Extraction - reduces sample preparation time by 70%
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| - | * Versatility - various selectivities available, adapts to any GC or HPLC system, can be automated
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| - | * Efficient & Economical - little/no use of solvents, quantitative method, allows more than 50 extractions per fiber on average
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| - | === Comparison of SPME to SPE ===
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| - | '''SPE''' exhaustively extracts all of the analytes, but they are diluted during elution 1mL of sample containing 1µg/mL of analyte passed through tube, eluted with 1mL of solvent = 1µg/mL X 1µl injection = 1ng (Concentrating to 100µl or injecting 10µl = 10ng on column
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| - | '''SPME''' partially extracts analytes but desorbs the entire amount 1mL sample containing 1µg/mL of analyte extracted with fiber. 1% goes on fiber. 0.01 x 1µg = 10ng desorbed on column
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| - | * [http://www.sigmaaldrich.com/analytical-chromatography/video/spme-video.html Sigma Aldrich SPME Website and information]
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| - | === Useful links ===
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| - | * [http://en.wikipedia.org/wiki/Gas_chromatography General Information on Gas Chromatography]
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| - | * [http://en.wikipedia.org/wiki/Solid_Phase_Microextraction Definition of SPME from Wikipedia]
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| | * [http://www.leaptec.com/contact-us.php Contact the LEAP office] | | * [http://www.leaptec.com/contact-us.php Contact the LEAP office] |
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| - | [[Category:Gas Chromatography]] | + | [[Category:MALDI]] |
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| - | [[Category:http://www.leapwiki.com/mediawiki/index.php?title=MALDI]]
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Revision as of 01:07, 12 May 2009
| MALDI Spotting with the PAL
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| Application Type
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| STANDARD
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| Application ID
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| MALDI Spotting
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| Description
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| MALDI Spotting with the PAL
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Abstract
Solid phase microextraction, or SPME, is a sample preparation technique used both in the laboratory and on-site. Developed in the early 1990s at the University of Waterloo by Dr. Pawliszyn's group, it is a simple and inexpensive technique where the use of solvents is not necessary.
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Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
