MALDI Spotting with the PAL

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(New page: {{LSApp |name = MALDI Spotting with the PAL |image = SPME_Twin_PAL.png |type = '''STANDARD''' |id = MALDI Spotting |description = MALDI Spotting with...)
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<gallery>
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   Image:Vial tray over a GC.jpg‎ | Sample vial tray
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   Image:4_plates_with_Tips.png| 4 Plates with Tips
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  Image:PAL head with magnetic attachment.jpg‎ | Magnetic tool tip
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   Image:ABI_Tray.png | ABI Plate
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  Image:PAL bar code reader.jpg‎ | Bar code reader
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  Image:PAL at Agitator position 1 (wide).jpg‎ | Agitator
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  Image:PAL at Agitator position 1 (tall).jpg‎ | Agitator
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  Image:Hanging vial.jpg‎ | Vial in transfer
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  Image:Agilent 6890 GC with 5975C MSD.jpg‎ | Agilent 6890 GC / 5975C VL MSD
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  Image:SPME_adapter_and_fibers.png | SPME Adapter and Fibers
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  Image:SPME_PAL.png | CTC Autosampler PAL with SPME
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  Image:SPME_Twin_PAL.png | CTC Twin PAL Autosampler with Sample Prep and SPME
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  Image:NDL1.png | Needle Heater Optional Accessory for SPME
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   Image:NDL2.png | Needle Heater Optional Accessory for SPME
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Revision as of 01:02, 12 May 2009

MALDI Spotting with the PAL
Application Type
  STANDARD
Application ID
  MALDI Spotting
Description
  MALDI Spotting with the PAL
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Contents

Abstract

Solid phase microextraction, or SPME, is a sample preparation technique used both in the laboratory and on-site. Developed in the early 1990s at the University of Waterloo by Dr. Pawliszyn's group, it is a simple and inexpensive technique where the use of solvents is not necessary.

Photos

4 Plates with Tips

ABI Plate

SPME offers some important advantages:

  • Single Step Extraction - reduces sample preparation time by 70%
  • Versatility - various selectivities available, adapts to any GC or HPLC system, can be automated
  • Efficient & Economical - little/no use of solvents, quantitative method, allows more than 50 extractions per fiber on average

Comparison of SPME to SPE

SPE exhaustively extracts all of the analytes, but they are diluted during elution 1mL of sample containing 1µg/mL of analyte passed through tube, eluted with 1mL of solvent = 1µg/mL X 1µl injection = 1ng (Concentrating to 100µl or injecting 10µl = 10ng on column

SPME partially extracts analytes but desorbs the entire amount 1mL sample containing 1µg/mL of analyte extracted with fiber. 1% goes on fiber. 0.01 x 1µg = 10ng desorbed on column


Useful links

www.leaptec.comwww.leapwiki.com
www.leaptec.com
www.leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information

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