M-005 Dual Channel Parallel Injection - LEAP Shell "(M-Fab)"

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{|  
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| Saving of Front and Back cut
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| Integration with Analyst
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|-
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| Performs "Look Ahead"
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| Single Sample List
 +
|-
 +
| Staggering of Samples
 +
|-
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| Multiplexes 2 separate LC systems into one Mass Spec
|-
|-
-
| Staggering of Injections
 
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{|  
{|  
|-
|-
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| Requires matching sample lists in Cycle Composer and Analyst
+
| Does not yet support 21CFR11
|-
|-
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| Columns may perform slightly different on alternating samples
+
| Requires Pumps to be integrated with LEAP Shell
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!width="15"|GLP  
!width="15"|GLP  
|- align=left
|- align=left
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| style="border:1px solid blue;"|'''M-003'''
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| style="border:1px solid blue;"|'''M-005'''
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| style="border:1px solid blue;"|'''Dual Channel Parallel Injection'''<br><br>Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition
+
| style="border:1px solid blue;"|'''Dual Channel Parallel Injection'''<br><br>Provides minimum cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition
-
| style="border:1px solid blue;"|Cycle Composer outside of Analyst
+
| style="border:1px solid blue;"|LEAP Shell
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| style="border:1px solid blue;"|* DoubleStack valve<br>*6 port injection valve<br>*FWS in front of 6 port valve<br>* Gradient Pump<br>* Regeneration pump
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| style="border:1px solid blue;"|*DoubleStack valve<br>*6 port injection valve<br>*FWS in front of 6 port valve<br>* Gradient Pump<br>* Regeneration pump
-
| style="border:1px solid blue;"|* Saving of Front and Back Cut<br>* Provides "Look Ahead"<br> *Provides staggering of injections
+
| style="border:1px solid blue;"|*Integration with Analyst Software<br>*Single Sample List<br> *Provides staggering of injections<br> *Multiplexes 2 separate LC systems into on MS
-
| style="border:1px solid blue;"|* Requires matching sample lists in Cycle Composer and Analyst<br>* Columns may perform slightly differently on alternating samples
+
| style="border:1px solid blue;"|* Does not support 21CFR11 compliance<br>*Requires pumps to be integrated with LEAP Shell
| style="border:1px solid blue;"|No
| style="border:1px solid blue;"|No
|}
|}

Revision as of 14:56, 30 April 2009

Dual Channel Parallel Injections with LEAP Shell "M-Fab"
Application Type
  SPECIAL
Application ID
  M-005
Description
  High Throughput LC injections alternating onto two analytical columns.
www.leaptec.comwww.leapwiki.com
www.leaptec.com
www.leapwiki.com

Contents

Overview

This configuration provides short cycle times by limiting the acquisition time to just the peaks of interest and regenerating the columns during the acquisition. It uses LEAP Shell Scheduling Software[1] - this application is integrated withAnalyst® Software[2] from Applied Biosystems Inc® . There is a single sample list and allows multiplexing [3] of 2 separate HPLC systems into one mass spectrometer.

  • LEAP Shell - M-Fab application integrated with Analyst with supported pumps.


If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.

Image:Front-backcut.png

If injections are staggered in the following way, there is a potential for up to 100% gain:


Image:Timeline_Dual_Stream_1.png

Hardware Requirements

  • DoubleStack multiple valve system and a 6 port injection valve with FWS in front of it is required
DoubleStack valve is used. Flow path intentionally left incomplete. Contact LEAP for more information
DoubleStack valve is used. Flow path intentionally left incomplete. Contact LEAP for more information





Key Benefits
Integration with Analyst
Single Sample List
Staggering of Samples
Multiplexes 2 separate LC systems into one Mass Spec



Restrictions
Does not yet support 21CFR11
Requires Pumps to be integrated with LEAP Shell







Code Application Control SW Key Hardware Key Benefits Restrictions GLP
M-005 Dual Channel Parallel Injection

Provides minimum cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition 		LEAP Shell *DoubleStack valve
*6 port injection valve
*FWS in front of 6 port valve
* Gradient Pump
* Regeneration pump 		*Integration with Analyst Software
*Single Sample List
*Provides staggering of injections
*Multiplexes 2 separate LC systems into on MS 		* Does not support 21CFR11 compliance
*Requires pumps to be integrated with LEAP Shell 		No


For other cost/time saving measures consider:

  • LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [4]
  • Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [5]
  • Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [6]
  • “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [7]
  • LEAP Shell Software and application for custom injections and scheduling [8]
www.leaptec.comwww.leapwiki.com
www.leaptec.com
www.leapwiki.com

Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information




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