M-005 Dual Channel Parallel Injection - LEAP Shell "(M-Fab)"
From LEAP
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=== Hardware Requirements === | === Hardware Requirements === | ||
- | * | + | * DoubleStack multiple valve system and a 6 port injection valve with FWS in front of it is required |
+ | [[Image:BlankDoubleand6port.png|frame|DoubleStack valve is used. Flow path intentionally left incomplete. Contact LEAP for more information]] | ||
+ | <br><br><br><br> | ||
+ | {| border="1" | ||
+ | | ''' Key Benefits ''' | ||
+ | |} | ||
+ | {| | ||
+ | |- | ||
+ | | Saving of Front and Back cut | ||
+ | |- | ||
+ | | Performs "Look Ahead" | ||
+ | |- | ||
+ | | Staggering of Injections | ||
+ | |} | ||
+ | <br><br> | ||
+ | {| border="1" | ||
+ | | '''Restrictions''' | ||
+ | |} | ||
+ | {| | ||
+ | |- | ||
+ | | Requires matching sample lists in Cycle Composer and Analyst | ||
+ | |- | ||
+ | | Columns may perform slightly different on alternating samples | ||
+ | |- | ||
+ | |} | ||
+ | <br><br><br><br><br><br> | ||
- | + | {| cellpadding="6" style="border:1px solid darkgray;" | |
+ | |- align=left | ||
+ | !width="20"|Code | ||
+ | !width="195"|Application | ||
+ | !width="85"|Control SW | ||
+ | !width="140"|Key Hardware | ||
+ | !width="205"|Key Benefits | ||
+ | !width="235"|Restrictions | ||
+ | !width="15"|GLP | ||
+ | |- align=left | ||
+ | | style="border:1px solid blue;"|'''M-003''' | ||
+ | | style="border:1px solid blue;"|'''Dual Channel Parallel Injection'''<br><br>Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition | ||
+ | | style="border:1px solid blue;"|Cycle Composer outside of Analyst | ||
+ | | style="border:1px solid blue;"|* DoubleStack valve<br>*6 port injection valve<br>*FWS in front of 6 port valve<br>* Gradient Pump<br>* Regeneration pump | ||
+ | | style="border:1px solid blue;"|* Saving of Front and Back Cut<br>* Provides "Look Ahead"<br> *Provides staggering of injections | ||
+ | | style="border:1px solid blue;"|* Requires matching sample lists in Cycle Composer and Analyst<br>* Columns may perform slightly differently on alternating samples | ||
+ | | style="border:1px solid blue;"|No | ||
+ | |} | ||
+ | <br> | ||
- | ----- | + | === For other cost/time saving measures consider:=== |
+ | * LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [http://www.leapwiki.com/mediawiki/index.php?title=Valve_Washing] | ||
+ | * Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-001_Dual_Channel_Serial_Injection_-_Analyst_with_ICC-CE] | ||
+ | * Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [http://www.leapwiki.com/mediawiki/index.php?title=M-002_Dual_Channel_Serial_Injection_with_front_cut_-_Analyst_with_ICC-CE] | ||
+ | * “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [http://www.leaptec.com/software/leap-shell-3.php] | ||
+ | * LEAP Shell Software and application for custom injections and scheduling [http://www.leaptec.com/software/leap-shell-3.php] | ||
+ | ---- | ||
[[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]] | [[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]] | ||
Revision as of 14:39, 30 April 2009
Dual Channel Parallel Injections with LEAP Shell "M-Fab" | |
Application Type | |
SPECIAL | |
Application ID | |
M-005 | |
Description | |
High Throughput LC injections alternating onto two analytical columns. |
Contents |
Overview
This configuration provides short cycle times by limiting the acquisition time to just the peaks of interest and regenerating the columns during the acquisition. It uses LEAP Shell Scheduling Software[1] - this application is integrated withAnalyst® Software[2] from Applied Biosystems Inc® . There is a single sample list and allows multiplexing [3] of 2 separate HPLC systems into one mass spectrometer.
- LEAP Shell - M-Fab application integrated with Analyst with supported pumps.
If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.
If injections are staggered in the following way, there is a potential for up to 100% gain:
Hardware Requirements
- DoubleStack multiple valve system and a 6 port injection valve with FWS in front of it is required
Key Benefits |
Saving of Front and Back cut |
Performs "Look Ahead" |
Staggering of Injections |
Restrictions |
Requires matching sample lists in Cycle Composer and Analyst |
Columns may perform slightly different on alternating samples |
Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
---|---|---|---|---|---|---|
M-003 | Dual Channel Parallel Injection Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition | Cycle Composer outside of Analyst | * DoubleStack valve *6 port injection valve *FWS in front of 6 port valve * Gradient Pump * Regeneration pump | * Saving of Front and Back Cut * Provides "Look Ahead" *Provides staggering of injections | * Requires matching sample lists in Cycle Composer and Analyst * Columns may perform slightly differently on alternating samples | No |
For other cost/time saving measures consider:
- LEAP offers multiple choices of Valve Self Wash Stations to aid in cleaning the valve [4]
- Dual Channel Serial Injection (M-001) using Analyst and ICC-CE [5]
- Dual Channel Serial Injection with Front Cut (M-002) using Analyst and ICC-CE [6]
- “Look Ahead”, sample prep before injection (M-004) using LEAP Shell Software [7]
- LEAP Shell Software and application for custom injections and scheduling [8]
Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information