M-003 Dual Channel Parallel Injection - Cycle Composer
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| - | + | {| cellpadding="6" style="border:1px solid darkgray;" | |
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| + | !width="20"|Code | ||
| + | !width="195"|Application | ||
| + | !width="85"|Control SW | ||
| + | !width="140"|Key Hardware | ||
| + | !width="205"|Key Benefits | ||
| + | !width="235"|Restrictions | ||
| + | !width="15"|GLP | ||
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| + | | style="border:1px solid blue;"|'''M-003''' | ||
| + | | style="border:1px solid blue;"|'''Dual Channel Parallel Injection'''<br><br>Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition | ||
| + | | style="border:1px solid blue;"|Cycle Composer outside of Analyst | ||
| + | | style="border:1px solid blue;"|* Trio VS<br>*6 port injection valve<br>*FWS in front of 6 port valve<br>* Gradient Pump<br>* Regeneration pump | ||
| + | | style="border:1px solid blue;"|* Saving of Front and Back Cut<br>* Provides "Look Ahead"<br> *Provides staggering of injections | ||
| + | | style="border:1px solid blue;"|* Requires matching sample lists in Cycle Composer and Analyst<br>* Columns may perform slightly differently on alternating samples | ||
| + | | style="border:1px solid blue;"|No | ||
| + | |} | ||
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[[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]] | [[Image:LEAP Logo.png|frame|www.leaptec.com<br>www.leapwiki.com]] | ||
Revision as of 23:13, 28 April 2009
| LC Dual Stream Staggered Injections |
| Application Type | |
| SPECIAL | |
| Application ID | |
| M-003 | |
| Description | |
| High Throughput LC injections alternating onto two analytical columns. |
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Overview
This configuration allows shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating the alternating column during the acquisition. It uses
Cycle Composer Software[1] - this application is run with Standard LEAP macros outside of Analyst Software[2] from Applied Biosystems Inc
If the peaks of interest come off together in the middle of the gradient (as shown below) then it is possible to utilize the front cut and back cut time to increase throughput.
If injections are staggered in the following way, there is a potential for up to 100% gain:
Hardware Requirements
- TrioVS multiple valve system is required in addition to the standard injection valve.
| Code | Application | Control SW | Key Hardware | Key Benefits | Restrictions | GLP |
|---|---|---|---|---|---|---|
| M-003 | Dual Channel Parallel Injection Provides shorter cycle times by limiting the acquisition time to just the peaks of interest and regenerating columns during acquisition | Cycle Composer outside of Analyst | * Trio VS *6 port injection valve *FWS in front of 6 port valve * Gradient Pump * Regeneration pump | * Saving of Front and Back Cut * Provides "Look Ahead" *Provides staggering of injections | * Requires matching sample lists in Cycle Composer and Analyst * Columns may perform slightly differently on alternating samples | No |
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Contact LEAP
For additional information about this technique please contact LEAP Technologies for detailed information
