LS-H1008 - Dow. Sample Cleanup and injection using Whatman Mini-Uniprep filters
From LEAP
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'''General Description of the Application''' | '''General Description of the Application''' | ||
| - | + | A fully automated sample prep system which is run unattended 24/7. Samples from a larger robotic system are presented in pairs to the PAL. The PAL is triggered by a contact closure to pick up the sample and read an associated information file. One of the sample pairs is filtered using the '''Whatman Mini-Uniprep filters''' . LEAP has developed a PRESS which automates this step. Sample pairs are injected onto two different valves each hooked up to a HPLC system controlled by EZChrom. | |
| - | A pair of 2oz sample bottles will be transferred into the tray holder by an existing robotic system | + | The PAL system is controlled by '''LEAP Shell''' (version 3.0). |
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| + | A pair of 2oz sample bottles will be transferred into the tray holder by an existing robotic system . One contains the assay sample and the other contains the HCB sample. The assay sample will be transferred into a 2 mL vial. The HCB sample will be transferred into a Mini-Uniprepchamber, PAL will pick the filter plunger and insert into the chamber and transfer them into the Mini-Uniprep processing device. After the filtration, the Mini-Uniprep vial will be brought back to the tray holder. Two LC systems are connected to the PAL, one for the assay sample and the other for HCB. Whenever either one of them is ready, the next prepared sample from the tray holder will be injected into the LC valve. A blank sister control from a 20 mL bottle will be processed before each assay sample. | ||
[[Image:LS-H1008_Dow_System_diagram.png]] | [[Image:LS-H1008_Dow_System_diagram.png]] | ||
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| + | All processing information will be written into an Excel output file. | ||
Revision as of 20:36, 4 February 2009
General Description of the Application
A fully automated sample prep system which is run unattended 24/7. Samples from a larger robotic system are presented in pairs to the PAL. The PAL is triggered by a contact closure to pick up the sample and read an associated information file. One of the sample pairs is filtered using the Whatman Mini-Uniprep filters . LEAP has developed a PRESS which automates this step. Sample pairs are injected onto two different valves each hooked up to a HPLC system controlled by EZChrom.
The PAL system is controlled by LEAP Shell (version 3.0).
A pair of 2oz sample bottles will be transferred into the tray holder by an existing robotic system . One contains the assay sample and the other contains the HCB sample. The assay sample will be transferred into a 2 mL vial. The HCB sample will be transferred into a Mini-Uniprepchamber, PAL will pick the filter plunger and insert into the chamber and transfer them into the Mini-Uniprep processing device. After the filtration, the Mini-Uniprep vial will be brought back to the tray holder. Two LC systems are connected to the PAL, one for the assay sample and the other for HCB. Whenever either one of them is ready, the next prepared sample from the tray holder will be injected into the LC valve. A blank sister control from a 20 mL bottle will be processed before each assay sample.
All processing information will be written into an Excel output file.
