LS-H1008 - Dow. Sample Cleanup and injection using Whatman Mini-Uniprep filters
From LEAP
(New page: General Description of the Application This HTS PAL will include a device to process Whatmen Mini-Uniprep filters, three standard tray holders, 5-position reagent holder for 10 and 20ml v...) |
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| - | General Description of the Application | + | '''General Description of the Application''' |
| - | This HTS PAL will include a device to process | + | This HTS PAL will include a device to process Whatman Mini-Uniprep filters, three standard tray holders, 5-position reagent holder for 10 and 20ml vials, two 6-port LC Valves and a fast wash station. One tray holder is for Mini-Uniprep filter plungers and liquid chambers, one tray holder for 2oz bottles and 20 mL bottles, and the other tray for 1mL bottles. '''LEAP Shell''' (version 3.0) will be used to run the application. |
A pair of 2oz sample bottles will be transferred into the tray holder by an existing robotic system separately. One contains the assay sample and the other contains the HCB sample. The assay sample will be transferred into a 2 mL vial. The HCB sample will be transferred into a Mini-Uniprepchamber, PAL will pick the filter plunger and insert into the chamber and transfer them into the Mini-Uniprep processing device. After the filtration, the Mini-Uniprep vial will be brought back to the tray holder. Two LC systems are connected to the PAL, one for the assay sample and the other for HCB. Whenever either one of them is ready, the next prepared sample from the tray holder will be injected into the LC valve. A blank sister control from a 20 mL bottle will be processed before each assay sample. | A pair of 2oz sample bottles will be transferred into the tray holder by an existing robotic system separately. One contains the assay sample and the other contains the HCB sample. The assay sample will be transferred into a 2 mL vial. The HCB sample will be transferred into a Mini-Uniprepchamber, PAL will pick the filter plunger and insert into the chamber and transfer them into the Mini-Uniprep processing device. After the filtration, the Mini-Uniprep vial will be brought back to the tray holder. Two LC systems are connected to the PAL, one for the assay sample and the other for HCB. Whenever either one of them is ready, the next prepared sample from the tray holder will be injected into the LC valve. A blank sister control from a 20 mL bottle will be processed before each assay sample. | ||
Revision as of 20:24, 4 February 2009
General Description of the Application
This HTS PAL will include a device to process Whatman Mini-Uniprep filters, three standard tray holders, 5-position reagent holder for 10 and 20ml vials, two 6-port LC Valves and a fast wash station. One tray holder is for Mini-Uniprep filter plungers and liquid chambers, one tray holder for 2oz bottles and 20 mL bottles, and the other tray for 1mL bottles. LEAP Shell (version 3.0) will be used to run the application.
A pair of 2oz sample bottles will be transferred into the tray holder by an existing robotic system separately. One contains the assay sample and the other contains the HCB sample. The assay sample will be transferred into a 2 mL vial. The HCB sample will be transferred into a Mini-Uniprepchamber, PAL will pick the filter plunger and insert into the chamber and transfer them into the Mini-Uniprep processing device. After the filtration, the Mini-Uniprep vial will be brought back to the tray holder. Two LC systems are connected to the PAL, one for the assay sample and the other for HCB. Whenever either one of them is ready, the next prepared sample from the tray holder will be injected into the LC valve. A blank sister control from a 20 mL bottle will be processed before each assay sample.
