ITEX-2

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(New page: {{LSApp |name = Multiple Headspace Extractions Option (MHE) |image = MHE1.png |type = '''Standard Product''' |id = Extend the headspace mode |descr...)
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{{LSApp
{{LSApp
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   |name        = Multiple Headspace Extractions Option (MHE)
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   |name        = ITEX-2
   |image      = MHE1.png  
   |image      = MHE1.png  
   |type        = '''Standard Product'''
   |type        = '''Standard Product'''
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{{logo}}
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=== Multiple Headspace Extractions Option (MHE) ===
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----
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The '''PAL Multiple Headspace Extraction (MHE)''' Option is an add-on module to extend the headspace mode of the LEAP CTC Analytics CombiPAL Robot. In classical headspace technique one sampling is done per vial. The quantitative determination can be inaccurate in the presence of interfering matrices or in cases where a calibration standard cannot be made with the exact same matrix composition. The MHE option consists of a venting tool combined with a Park station. The MHE technique requires a series of sampling cycles using the same vial. First, a sample is pressurized to a specific pressure. The vial is then sampled and vented. The vial is re-pressurized and sampled again. This process is repeated multiple times to obtain final results.
 
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MHE is generally regarded as one of the methods of choice with analysis of difficult headspace samples.Matrix effects are practically eliminated with MHE and the difficulties of quantitative headspace analysis can be overcome.
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=== ITEX-2 Option ===
-
Multiple headspace extraction involves a sequence of pressurizing, sampling, and venting from the sample for each of the extraction steps. Traditionally, the pressurizing and venting steps are difficult to control with a syringe‐based system.
+
The '''ITEX-2''' Option consists of an add-on module which can be used with any existing or new CombiPAL robot. It performs enrichment of volatile or semi-volatile compounds during headspace analysis. A microtrap filled with adsorbent material, such as Tenax or activated charcoal is placed between the heated CombiPAL Headspace syringe and the syringe needle. No cryofocussing is needed to obtain sharp peaks. The ITEX-2 Option offers faster desorption, a complete deactivated sample path and a shortened syringe. Adding ITEX to your existing COMBI PAL autosampler is now as easy as switching from Liquid to Headspace Sampling. The ITEX-2 is an alternative to traditional purge and trap in many cases.
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A simple tool was manufactured, allowing for the controlled venting of the pressurized sample vial after each sample extraction step. The operation of the tool is fully integrated into the Cycle Composer software used to control the Combi PAL robot. The pressurization is done using the built‐in standard functions of the CombiPAL autosampler.
+
 +
* Rapid and efficient sample enrichment of volatile and semi-volatile compounds in solid, liquid and gaseous samples
 +
 +
* In-tube extraction and direct thermal desorption using proven industry standard adsorbents (Tenax TA, Carbotrap, Carbosieve etc.)
 +
 +
* Syringe only concept for transparent sample handling, no sample loops, transfer lines, or switching valves
 +
 +
* Top mounted on GC's, saves valuable bench space
 +
 +
* No GC injector modifications, no cryo-focussing necessary
 +
 +
* Interfaces with any CombiPAL System, controlled by all major GC/GC-MS
 +
 +
=== Significant Markets  ===
 +
* Environmental
 +
* Drinking Water
 +
* Foods / Flavors
 +
* Consumer Products
 +
* Forensics / Toxicology
 +
* Petrochemicals
 +
* Polymers
 +
* Pharmaceuticals
 +
* Residual Solvents
 +
 +
=== Steps of CombiPAL ITEX Cycle ===
 +
'''1''' Sample is heated and / or agitated in a sealed sample vial until equilibrium is achieved <br>                     
 +
'''2''' The Trap needle pierces the vial septa and the syringe pumps the headspace through the Trap<br>   
 +
'''3''' The loaded ITEX Trap is flash heated up to 350°C and desorbed into the hot GC Injector<br>   
 +
'''4''' After thermal desorption the hot ITEX Trap is cleaned with inert flush gas<br>   
 +
 +
[[Image:ITEX_steps.png‎]]
 +
 +
 +
'''Extraction Trap:'''
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44 mg adsorbent Tenax TA 80/100 mesh
 +
<br>
 +
'''Pumping syringe:'''
 +
1.3ml HD syringe with removable trap
 +
<br>
 +
'''Extraction speed:'''
 +
10µl/sec. - 1000µl/sec
 +
<br>
 +
'''Extraction strokes:'''
 +
1 -999
 +
<br>
 +
'''Extraction volume:'''
 +
130µl - 1300 µl per stroke
 +
<br>
 +
'''Desorption temp:'''
 +
+5°C above ambient – 350°C selectable in 1°C steps
 +
<br>
 +
'''Desorption time:'''
 +
0 - 300 sec.
 +
<br>
 +
'''Desorption speed:'''
 +
1µl/s - 500µl/s
 +
<br>
 +
'''Heating-up rate:'''
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up to 12°C/s
 +
<br>
 +
'''Trap cleaning:'''
 +
Inert gas purging, 30 - 600 sec.
 +
<br>
 +
'''Sample capacity:'''
 +
up to 297 2ml standard vials
 +
up to 96 10ml or 20ml vials
 +
optional Cooled Trayholders available
 +
<br>
 +
'''Incubator oven:'''
 +
6 heated positions for 2ml/10ml/20ml vials
 +
<br>
 +
'''Incubation temp:'''
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30°C – 200°C selectable in 1°C steps
 +
<br>
 +
'''Incubation time:'''
 +
up to 999 min.
 +
<br>
 +
'''Agitation:'''
 +
Interval shaking 250rpm – 750 rpm prior sample extraction
 +
<br>
=== Photos ===
=== Photos ===
<gallery>
<gallery>
-
   Image:MHE1.png | MHE option on PAL
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   Image:ITEX.png | ITEX
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   Image:MHE2a.png | The sample is withdrawn from the vial
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   Image:Itex1.png| ITEX Extraction Step
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   Image:MHE2b.png | The venting tool is positioned above the sample vial
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   Image:Itex_2.png |ITEX Desorption Step
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   Image:MHE2c.png | Activating the venting tool vents the vial content to ambient
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   Image:Itex_3.png |ITEX Trap
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  Image:MHE3.png | Injecting headspace sample onto GC
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</gallery>
</gallery>
 +
 +
 +
=== Additional Information ===
 +
 +
The PAL ITEX-2 Option is an add-on module to extend the range of headspace techniques. ITEX can be considered an automated dynamic headspace technique. In classical headspace technique one sampling is done per vial. In this device, analytes are adsorbed (loaded) on a sorbent trap contained within a side-hole needle attached to a gas-tight syringe. Following this loading procedure analytes are thermally desorbed and led directly into the GC injection port, then analyzed by the required technique, mainly using GC-MS detection.<br>
 +
Loading takes place by continuously moving the headspace syringe plunger up and down, passing the headspace gas phase that exists above a sample through the sorbent trap inside the needle numerous times.<br>
 +
Dynamic headspace extraction increases the concentration of analyte in order to extend the range of detection as compared to conventional static headspace techniques. Required levels of detection can be reached by applying more pumping (up and down) cycles. To reach the detection limit in the range of a ppm to ppb concentration, three to twenty strokes are typically applied and, for the ppt-level of concentration, 40 to 60 pumping strokes may be necessary.<br>
 +
The PAL ITEX-2 Option consists of a dedicated gas tight syringe with an adapter for a replaceable needle and a heated syringe cartridge. The side-hole needle includes an integrated trap (standard trap material is Tenax TA) and a trap/desorber heater<br>
 +
The only recommended application for the ITEX sample enrichment technique is from the gas phase, the headspace in the vial<br>
 +
 +
=== Hardware Requirements ===
 +
The PAL ITEX-2 option can be used with any PAL System equipped for static headspace technique, e.g. Combi PAL. The revision level of the PAL Injection Unit must be Rev. E or higher, slot at left side is required. Example: PNo.: MZ 02-00 Revision E.
=== Downloads ===
=== Downloads ===
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The MHE technique is demonstrated with the CombiPAL, a syringe based autosampler. The quality of the data compares well with those obtained from a loop-based sampling system. Please see Poster:
 
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[[image:Download Icon.png|40px]] [[:Image:Mhe-poster.pdf | Poster on MHE by Tore Vulpius and Bruno Baltensperger]]
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[[image:Download Icon.png|40px]] [[Media:ITEX-2_LEAPBrochure.pdf | ITEX-2 Brochure]]<br>
 +
[[image:Download Icon.png|40px]] [[Media:ITEX-ApplicationBinder_000.pdf | ITEX Application Binder]]
=== PRINT Function for Page ===
=== PRINT Function for Page ===
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[[image:Download Icon.png|40px]] [[:Image:Print_of_MHE_on_LEAPWiki.pdf | PDF of this page formatted for printing]]
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[[image:Download Icon.png|40px]] [[Media:Print_of_ITEX-2_for_LEAP_Wiki.pdf | PDF of this page formatted for printing]]
=== Accessories for the PAL ===
=== Accessories for the PAL ===
[[image:Accessories_Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Category:Accessories Other Accessories for PAL Robots]
[[image:Accessories_Icon.png|40px]] [http://www.leapwiki.com/mediawiki/index.php?title=Category:Accessories Other Accessories for PAL Robots]
-
 
+
=== Videos of PAL ===
 +
[[Image:Movie Icon.png|40px]][http://www.youtube.com/user/LEAPTechnologies#g/u LEAP's PAL Application Videos on YouTube]
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-----
{{logo}}
{{logo}}
LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis. <br>
LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis. <br>
-
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
+
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions. Check with your LEAP representative to find out if teh ITEX-2 is a suitable alternative to your purge and trap methods.
=== Contact LEAP ===
=== Contact LEAP ===

Current revision

ITEX-2
Application Type
  Standard Product
Application ID
  Extend the headspace mode
Description
  Accessory Showcase
 ↳ leaptec.com  ↳ leapwiki.com
 ↳ leaptec.com
 ↳ leapwiki.com


Contents

ITEX-2 Option

The ITEX-2 Option consists of an add-on module which can be used with any existing or new CombiPAL robot. It performs enrichment of volatile or semi-volatile compounds during headspace analysis. A microtrap filled with adsorbent material, such as Tenax or activated charcoal is placed between the heated CombiPAL Headspace syringe and the syringe needle. No cryofocussing is needed to obtain sharp peaks. The ITEX-2 Option offers faster desorption, a complete deactivated sample path and a shortened syringe. Adding ITEX to your existing COMBI PAL autosampler is now as easy as switching from Liquid to Headspace Sampling. The ITEX-2 is an alternative to traditional purge and trap in many cases.

  • Rapid and efficient sample enrichment of volatile and semi-volatile compounds in solid, liquid and gaseous samples
  • In-tube extraction and direct thermal desorption using proven industry standard adsorbents (Tenax TA, Carbotrap, Carbosieve etc.)
  • Syringe only concept for transparent sample handling, no sample loops, transfer lines, or switching valves
  • Top mounted on GC's, saves valuable bench space
  • No GC injector modifications, no cryo-focussing necessary
  • Interfaces with any CombiPAL System, controlled by all major GC/GC-MS

Significant Markets

  • Environmental
  • Drinking Water
  • Foods / Flavors
  • Consumer Products
  • Forensics / Toxicology
  • Petrochemicals
  • Polymers
  • Pharmaceuticals
  • Residual Solvents

Steps of CombiPAL ITEX Cycle

1 Sample is heated and / or agitated in a sealed sample vial until equilibrium is achieved
2 The Trap needle pierces the vial septa and the syringe pumps the headspace through the Trap
3 The loaded ITEX Trap is flash heated up to 350°C and desorbed into the hot GC Injector
4 After thermal desorption the hot ITEX Trap is cleaned with inert flush gas

Image:ITEX_steps.png‎


Extraction Trap: 44 mg adsorbent Tenax TA 80/100 mesh
Pumping syringe: 1.3ml HD syringe with removable trap
Extraction speed: 10µl/sec. - 1000µl/sec
Extraction strokes: 1 -999
Extraction volume: 130µl - 1300 µl per stroke
Desorption temp: +5°C above ambient – 350°C selectable in 1°C steps
Desorption time: 0 - 300 sec.
Desorption speed: 1µl/s - 500µl/s
Heating-up rate: up to 12°C/s
Trap cleaning: Inert gas purging, 30 - 600 sec.
Sample capacity: up to 297 2ml standard vials up to 96 10ml or 20ml vials optional Cooled Trayholders available
Incubator oven: 6 heated positions for 2ml/10ml/20ml vials
Incubation temp: 30°C – 200°C selectable in 1°C steps
Incubation time: up to 999 min.
Agitation: Interval shaking 250rpm – 750 rpm prior sample extraction

Photos

ITEX

ITEX Extraction Step

ITEX Desorption Step

ITEX Trap


Additional Information

The PAL ITEX-2 Option is an add-on module to extend the range of headspace techniques. ITEX can be considered an automated dynamic headspace technique. In classical headspace technique one sampling is done per vial. In this device, analytes are adsorbed (loaded) on a sorbent trap contained within a side-hole needle attached to a gas-tight syringe. Following this loading procedure analytes are thermally desorbed and led directly into the GC injection port, then analyzed by the required technique, mainly using GC-MS detection.
Loading takes place by continuously moving the headspace syringe plunger up and down, passing the headspace gas phase that exists above a sample through the sorbent trap inside the needle numerous times.
Dynamic headspace extraction increases the concentration of analyte in order to extend the range of detection as compared to conventional static headspace techniques. Required levels of detection can be reached by applying more pumping (up and down) cycles. To reach the detection limit in the range of a ppm to ppb concentration, three to twenty strokes are typically applied and, for the ppt-level of concentration, 40 to 60 pumping strokes may be necessary.
The PAL ITEX-2 Option consists of a dedicated gas tight syringe with an adapter for a replaceable needle and a heated syringe cartridge. The side-hole needle includes an integrated trap (standard trap material is Tenax TA) and a trap/desorber heater
The only recommended application for the ITEX sample enrichment technique is from the gas phase, the headspace in the vial

Hardware Requirements

The PAL ITEX-2 option can be used with any PAL System equipped for static headspace technique, e.g. Combi PAL. The revision level of the PAL Injection Unit must be Rev. E or higher, slot at left side is required. Example: PNo.: MZ 02-00 Revision E.

Downloads

ITEX-2 Brochure
ITEX Application Binder

PRINT Function for Page

PDF of this page formatted for printing

Accessories for the PAL

Other Accessories for PAL Robots

Videos of PAL

LEAP's PAL Application Videos on YouTube

 ↳ leaptec.com  ↳ leapwiki.com
 ↳ leaptec.com
 ↳ leapwiki.com

LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions. Check with your LEAP representative to find out if teh ITEX-2 is a suitable alternative to your purge and trap methods.

Contact LEAP

Contact LEAP

For additional information about LEAP and the PAL Platform, please contact LEAP Technologies.

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