H1108 - Merck. Multiple columns and MS Tuning in Analyst

From LEAP

Dr. Larry Colwell will receive an HTS PAL with a Cool Stack, a fast wash station, a 6-port injection valve and a TrioVS (6-port, 10-port, selector). There are four Analyst cycles that will be slight variations of the standard LC injection cycle.

The first cycle is known as a Tuning cycle. It collect samples from the VT-54 tray and passes them directly to the Mass Spec through the stand-alone 6-port LC Valve. By directly, it is meant to emphasize that the sample does not pass through any column whatsoever during its passage to the Mass Spec.

The second and third Analyst cycles inject the sample to the top valve of the TrioVS. The difference between the two cycles is that one passes the sample through column A (connected to ports 10 and 7 of the 2nd Valve of the TrioVS) on its way to the Mass Spec, while the other cycle passes the sample through column B (connected to ports 2 and 5 of the 2nd Valve of the TrioVS).

The final Analyst cycle is used for a single-column application. In this scenario, the second valve of the TrioVS is bypassed altogether. The column will be included in the plumbing lines connecting the 6-port valve of the TrioVS to the selector valve of the TrioVS.