Dual Channel Sample Cleanup
From LEAP
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4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column. | 4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column. | ||
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| + | [[Image:Dual_Channel_Sample_Filtration_PlumbDiag.png]] | ||
'''Project Ref: H1073b''' | '''Project Ref: H1073b''' | ||
Revision as of 20:30, 26 March 2009
General Description of process:
Samples are injected and pass through a guard column which is in-line with the analytical column. At a specified time, the guard column switches out of line and is regenerated using a wash pump configured to do a step gradient while the sample peaks are acquired.
To increase throughput, samples are injected ahead of the acquisition time, so that pre-filtration is occurring concurrently with the previous samples acquisition. Each line of the sample list processes a pair of samples which are staggered in this way (see time chart at the end of this document).
4 pumps are required: - 2 wash pumps (Agilent 1100) pre-programmed to perform the step gradient and 2 isocratic analytical pumps running continuously one for each column.
Project Ref: H1073b
