Multi Headspace Extractions
From LEAP
| Multiple Headspace Extractions Option (MHE) |
| Application Type | |
| Standard Product | |
| Application ID | |
| Extend the headspace mode | |
| Description | |
| Accessory Showcase |
Contents |
Multiple Headspace Extractions Option (MHE)
The PAL Multiple Headspace Extraction (MHE) Option is an add-on module to extend the headspace mode of the LEAP CTC Analytics COMBI PAL Robot. In classical headspace technique one sampling is done per vial. The quantitative determination can be inaccurate in the presence of interfering matrices or in cases where a calibration standard cannot be made with the exact same matrix composition. The MHE option consists of a venting tool combined with a Park station. The MHE technique requires a series of sampling cycles using the same vial. First, a sample is pressurized to a specific pressure. The vial is then sampled and vented. The vial is re-pressurized and sampled again. This process is repeated multiple times to obtain final results.
MHE is generally regarded as one of the methods of choice with analysis of difficult headspace samples.Matrix effects are practically eliminated with MHE and the difficulties of quantitative headspace analysis can be overcome.
Multiple headspace extraction involves a sequence of pressurizing, sampling, and venting from the sample for each of the extraction steps. Traditionally, the pressurizing and venting steps are difficult to control with a syringe‐based system. A simple tool was manufactured, allowing for the controlled venting of the pressurized sample vial after each sample extraction step. The operation of the tool is fully integrated into the Cycle Composer software used to control the COMBI PAL robot. The pressurization is done using the built‐in standard functions of the CombiPAL autosampler.
Photos
MHE option on PAL |
 The sample is withdrawn from the vial |
 The venting tool is positioned above the sample vial |
 Activating the venting tool vents the vial content to ambient |
 Injecting headspace sample onto GC |
 Tool with needle shown |
Downloads
The MHE technique is demonstrated with the CombiPAL, a syringe based autosampler. The quality of the data compares well with those obtained from a loop-based sampling system. Please see Poster:
Poster on MHE by Tore Vulpius and Bruno Baltensperger
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Accessories for the PAL
Other Accessories for PAL Robots
LEAP strives to find total solutions for analytical lab automation by automating analytical processes for small and large molecules in extracted liquids, solids, and recently in human, animal and plant tissues. We provide the precise robotics and efficient sample prep required by modern measurement techniques such as MALDITOF mass spectroscopy. Our newest specialty customization of CTC Analytics PAL features small workstations that can perform complex liquid handling tasks including HPLC-Purification, SPE, filtration, weighing, heating and stirring. They can be configured as stand alone units or integrated for “just in time” sample prep for LC-MS or GC-MS analysis.
LEAP provides automated workstation instrumentation solutions based on the LEAP CTC PAL X, Y, Z syringe only autosampler robot from LEAP Technologies. This extremely flexible, precise, and adaptable liquid handling robotic platform is available in a variety of lengths and options depending on the requirements of your sample preparation and injections for your UHPLC, LC or GC chromatography.LEAP offers full support and service for the PAL platform in addition to being able to write custom macros, cycles, and scheduling to your applications. Please contact LEAP Technologies on how we can help you get maximized throughput with flexible pipetting automation solutions.
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For additional information about LEAP and the PAL Platform, please contact LEAP Technologies. |
