Sample Filtration

From LEAP

	Sample Filtration
	Application Type
	SPECIAL
	Application ID
	PALPress
	Description
	Sample Filtration with Whatman Mini-Uniprep filter system

Overview

Whatman Mini Uniprep syringeless filters offer a convenient way of filtering samples containing particulates prior to introduction on an HPLC system. They come with 2 pore sizes and many different filter media [1]. They are designed for manual operation but are not conducive to large batch sizes - repeated pressing by hand being very fatiguing.

LEAP has developed a "Press" accessory for the PAL which allows samples to be filtered reliably and repeatably. It does not require external pneumatics, but uses the PAL Z motion itself to compress the filter chambers. Samples can be processes in batch mode or "just in time" before direct injection to the LC valve.

The PAL offers precise control of the downforce on the sample. The user can specify the speed of compression in the method, allowing even very heavy suspensions to be filtered successfully.

Significant Markets

Any labs who are doing High Performance Liquid Chromatography (HPLC) or more significantly, Ultra High Performance Liquid Chromatography (UHPLC) analysis. It is imperative that particles are eliminated to avoid blockage of HPLC lines and columns. This filtering technique let you prepare samples in one third the time required by other methods. Automation of the filtering step has NOT been possible in a reliable fashion till now.

Useful Links

Movie of the Press in action: Image:FilterPALhigh.mov