SPME

From LEAP

Revision as of 22:37, 4 May 2009

	GC SPME
	Application Type
	STANDARD
	Application ID
	GCSPME
	Description
	GC SPME

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Abstract

A simple and fairly standardized technique of extracting volatile and semi-volatile substances from raw samples in liquid or solid form is headspace analyzes [1]. A sample is placed in a vial that is sealed by a septum and cap and heated in an incubator for a specific time. Once the content of the vial has reached equilibrium a sampled is extracted and injected into the injection port of a GC. LEAP’s specific equipment the CTC COMBI PAL performs all the tasks for this extraction method unattended. For a sequence of samples up to 96 the COMBI PAL will schedule the vials to be in the incubator/agitator for the same amount of time before a precise sample of headspace gas is extracted by a heated syringe system for a subsequent appropriate injection into a GC. Many variables are programmable over a validated range. 1ml, 2.5ml and 5ml syringes and a solvent-less solid phase micro extraction system are the tools that can be mounted in the injection head.

Screenshots

LEAP Shell Method

Sample queue

PAL sample prep method

PAL sample injection method

Photos

Sample vial tray	Magnetic tool tip	Bar code reader	Agitator
Agitator	Vial in transfer	Agilent 6890 GC / 5975C VL MSD

SPME offers some important advantages:

1. Single Step Extraction - reduces sample preparation time by 70%
2. Versatility - various selectivities available, adapts to any GC or HPLC system, can be automated
3. Efficient & Economical - little/no use of solvents, quantitative method, allows more than 50 extractions per fiber on average

• [2]

Useful links

• General Information on Gas Chromatography

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Contact LEAP

For additional information about this technique please contact LEAP Technologies for detailed information

• Contact the LEAP office